Phylogenomic diversity of archigregarine apicomplexans.

IF 4.5 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Open Biology Pub Date : 2024-09-01 Epub Date: 2024-09-25 DOI:10.1098/rsob.240141
Gordon Lax, Eunji Park, Ina Na, Victoria Jacko-Reynolds, Waldan K Kwong, Chloe S E House, Morelia Trznadel, Kevin Wakeman, Brian S Leander, Patrick Keeling
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Abstract

Gregarines are a large and diverse subgroup of Apicomplexa, a lineage of obligate animal symbionts including pathogens such as Plasmodium, the malaria parasite. Unlike Plasmodium, however, gregarines are poorly studied, despite the fact that as early-branching apicomplexans they are crucial to our understanding of the origin and evolution of all apicomplexans and their parasitic lifestyle. Exemplifying this, the earliest branch of gregarines, the archigregarines, are particularly poorly studied: around 80 species have been described from marine invertebrates, but almost all of them were assigned to a single genus, Selenidium. Most are known only from light micrographs and largely unresolved rDNA phylogenies, where they exhibit a great deal of sequence variation, and fall into four subclades. To resolve the relationships within archigregarines, we sequenced 12 single-cell transcriptomes from species representing all four known subclades, as well as one blastogregarine (which frequently branch with Selenidium). A 190-gene phylogenomic tree confirmed four maximally supported individual clades of archigregarines and blastogregarines. These clades are discrete and distantly related, and also correlate with host identity. We propose the establishment of three novel genera of archigregarines to reflect their phylogenetic diversity and host range, and nine novel species isolated from a range of marine invertebrates.

弧菌的系统发生组多样性。
疟原虫(Gregarines)是拟疟原虫(Apicomplexa)的一个庞大而多样化的亚群,拟疟原虫是包括疟原虫(疟疾寄生虫)等病原体在内的强制性动物共生体。然而,与疟原虫不同的是,尽管作为早期分支的类囊虫对我们了解所有类囊虫的起源和进化及其寄生生活方式至关重要,但人们对加里宁虫的研究却很少。作为这方面的一个例证,对最早的革囊虫分支--古革囊虫的研究尤其薄弱:从海洋无脊椎动物中描述了大约 80 个物种,但几乎所有这些物种都被归入一个属--硒虫属。大多数物种只能从光照显微照片和基本未解决的 rDNA 系统进化论中得知,它们的序列变异很大,可分为四个亚支系。为了解决弓形虫内部的关系问题,我们对代表所有四个已知亚支系的物种以及一个爆裂弓形虫(经常与硒弓形虫分支)的 12 个单细胞转录组进行了测序。一棵190个基因的系统发生树确认了4个最大支持的弓形孢子虫和囊孢子虫支系。这些支系离散且亲缘关系较远,还与宿主身份相关。我们建议建立弓形虫的三个新属,以反映其系统发育的多样性和宿主范围,以及从一系列海洋无脊椎动物中分离出的九个新种。
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来源期刊
Open Biology
Open Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
10.00
自引率
1.70%
发文量
136
审稿时长
6-12 weeks
期刊介绍: Open Biology is an online journal that welcomes original, high impact research in cell and developmental biology, molecular and structural biology, biochemistry, neuroscience, immunology, microbiology and genetics.
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