Extraction of camptothecin and 10-hydroxycamptothecin from Camptotheca acuminata fruits by natural deep eutectic solvent.

IF 1.9 3区化学 Q3 CHEMISTRY, APPLIED

Natural Product Research Pub Date : 2024-10-01 DOI:10.1080/14786419.2024.2411363

Beibei Fang, Qing Liu, Chengli Yang, Kaixuan Liu, Guanya He, Xuchun Zhai, Kaishun Hu, Zulekha, Dali Li

引用次数: 0

Abstract

A natural deep eutectic solvent-based ultrasound-assisted simultaneous extraction (NADES-UAE) of camptothecin (CPT) and 10-hydroxycamptothecin (10-HCPT) was established. The 1.31 mg of CPT and 1.66 mg of 10-HCPT were obtained from each gram of the fruit powder of Camptotheca acuminata under the optimum conditions with a water content of 20%, a liquid-solid ratio of 12 mL/g and an ultrasound time of 20 min. The recovery efficiencies of CPT and 10-HCPT after AB-8 resin enrichment were 70.5% and 74.8%, respectively. The stronger interaction between NADES3 which was screened from 12 kinds of NADES and target components compared with methanol or water was demonstrated using molecular dynamics simulation. Moreover, the recovered NADES3 could be reused at least 4 times. The present research provided an efficient, environment-friendly, and sustainable method for extracting and recovering CPT and 10-HCPT from the fruits of C. acuminata.

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用天然深共晶溶剂萃取喜树果实中的喜树碱和 10-羟基喜树碱。

建立了一种基于天然深共晶溶剂的超声辅助同时萃取喜树碱（CPT）和10-羟基喜树碱（10-HPT）的方法（NADES-UAE）。在含水量为20%、液固比为12 mL/g、超声时间为20 min的最佳条件下，从每克喜树果粉中提取了1.31 mg CPT和1.66 mg 10-HPT。经 AB-8 树脂富集后，CPT 和 10-HCPT 的回收率分别为 70.5% 和 74.8%。分子动力学模拟证明，与甲醇或水相比，从 12 种 NADES 中筛选出的 NADES3 与目标成分之间的相互作用更强。此外，回收的 NADES3 可重复使用至少 4 次。本研究为从刺五加果实中提取和回收 CPT 和 10-HCPT 提供了一种高效、环保和可持续的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.