A method for quantitative and base-resolution sequencing of pseudouridine

IF 36.1 1区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Nature Methods Pub Date : 2024-09-30 DOI:10.1038/s41592-024-02466-5

引用次数: 0

Abstract

Quantitative and sensitive methods for the detection of pseudouridine (Ψ) have been lacking. Now, a method termed 2-bromoacrylamide-assisted cyclization sequencing (BACS) has been developed that enables the accurate quantification of Ψ stoichiometry, precise identification of Ψ positions and robust detection of densely modified Ψ sites.

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对假尿苷进行定量和碱基分辨率测序的方法。

一直以来，人们缺乏定量和灵敏的方法来检测假尿嘧啶（Ψ）。现在，一种被称为 2-溴丙烯酰胺辅助环化测序（BACS）的方法已经开发出来，它能够准确量化Ψ的化学计量，精确识别Ψ的位置，并对密集修饰的Ψ位点进行稳健的检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Methods 生物-生化研究方法

CiteScore

58.70

自引率

1.70%

发文量

326

审稿时长

1 months

期刊介绍： Nature Methods is a monthly journal that focuses on publishing innovative methods and substantial enhancements to fundamental life sciences research techniques. Geared towards a diverse, interdisciplinary readership of researchers in academia and industry engaged in laboratory work, the journal offers new tools for research and emphasizes the immediate practical significance of the featured work. It publishes primary research papers and reviews recent technical and methodological advancements, with a particular interest in primary methods papers relevant to the biological and biomedical sciences. This includes methods rooted in chemistry with practical applications for studying biological problems.