Establishment of reference intervals of haematology and biochemistry analytes in ICR mice of different ages.

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Suresh Patel, Satish Patel, Ashvin Kotadiya, Samir Patel, Bhavesh Shrimali, Tushar Patel, Harshida Trivedi, Vishal Patel, Jogeswar Mahapatra, Mukul Jain
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Abstract

Outbred stocks of mice are widely used in pre-clinical research as these animals possess a diversified genetic background when compared with inbred strains of mice. It is crucial to assess particular alterations in the physiological and functional profiles of laboratory animals using haematological and biochemical indicators. These values can also differ between laboratories because they are influenced by many different factors. We aimed to provide normal values and reference intervals for selected haematology and biochemistry analytes of 570 ICR mice at three different ages: 6-8 weeks, 10-14 weeks and 6-9 months. Reference values were calculated by non-parametric methods. For comparisons between sexes, the independent-sample t-test and Mann-Whitney test were employed, and analysis of variance was used for age differences. The findings of the study revealed age-related declines in haemoglobin concentration, haematocrit, mean corpuscular volume and mean corpuscular haemoglobin concentrations. Mice aged 6-9 months had statistically higher platelet counts in their blood than mice of other ages. The white blood cell count had a significant age effect and progressively decreased with age. As mice get older, the percentage of neutrophils, monocytes and basophils increases, but the percentage of lymphocytes decreases. For the biochemical values, age-related significant differences in glucose, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and albumin concentrations were found. It was also found that creatinine concentrations were comparable across all age ranges. The values presented in the present work can be used as a reference to interpret clinical pathology data for other studies and to evaluate health status.

确定不同年龄 ICR 小鼠血液学和生物化学分析指标的参考区间。
与近交系小鼠相比,近交系小鼠具有多样化的遗传背景,因此被广泛用于临床前研究。使用血液学和生化指标评估实验动物生理和功能特征的特定变化至关重要。由于受到许多不同因素的影响,这些数值在不同实验室之间也可能存在差异。我们的目标是为 570 只 ICR 小鼠在三个不同年龄段的选定血液学和生物化学分析指标提供正常值和参考区间:6-8周、10-14周和6-9个月。参考值采用非参数方法计算。性别间的比较采用独立样本 t 检验和曼-惠特尼检验,年龄差异采用方差分析。研究结果显示,血红蛋白浓度、血细胞比容、平均血球容积和平均血红蛋白浓度的下降与年龄有关。据统计,6-9 个月大的小鼠血液中血小板计数高于其他年龄段的小鼠。白细胞计数有明显的年龄效应,并随着年龄的增长而逐渐减少。随着小鼠年龄的增长,中性粒细胞、单核细胞和嗜碱性粒细胞的比例会增加,但淋巴细胞的比例会下降。在生化值方面,发现葡萄糖、天门冬氨酸氨基转移酶、丙氨酸氨基转移酶、碱性磷酸酶和白蛋白的浓度存在与年龄相关的显著差异。研究还发现,各年龄段的肌酐浓度相当。本研究提供的数值可作为其他研究解释临床病理学数据和评估健康状况的参考。
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来源期刊
Laboratory Animals
Laboratory Animals 生物-动物学
CiteScore
4.90
自引率
8.30%
发文量
64
审稿时长
6-12 weeks
期刊介绍: The international journal of laboratory animal science and welfare, Laboratory Animals publishes peer-reviewed original papers and reviews on all aspects of the use of animals in biomedical research. The journal promotes improvements in the welfare or well-being of the animals used, it particularly focuses on research that reduces the number of animals used or which replaces animal models with in vitro alternatives.
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