Anti-inflammatory and anti-arthritic activities of ethanolic extract of Myxopyrum serratulum A.W. Hill.

IF 2.7 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Sheela Rani T, Srikanth Jeyabalan, Sivaraman Dhanasekaran, Mahendran Sekar, Vetriselvan Subramaniyan, Ling Shing Wong
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Abstract

Background: Rheumatoid arthritis (RA) is a debilitating inflammatory disorder characterized by an overactive immune system targeting joints, leading to inflammation and intense pain. While current RA therapies effectively alleviate symptoms, they are often associated with significant side effects. This study aimed to assess the anti-inflammatory and anti-arthritic properties of an Ethanolic Extract of Myxopyrum serratulum A.W. Hill (EEMS) using animal models.

Results: The acute toxicity study with EEMS (2000 mg/kg, p.o.) on rats showed no toxicity or mortality up to the highest dose. Inflammation was induced using carrageenan, and rats were treated with varying doses of EEMS (100, 200, and 400 mg/kg, p.o.) and diclofenac to assess anti-inflammatory effects. Anti-arthritic efficacy was evaluated using Complete Freund's adjuvant (CFA)-induced inflammation, comparing EEMS to methotrexate. The results revealed dose-dependent anti-inflammatory effects of EEMS and a reversal of arthritic-induced weight loss in treated groups. Paw volume reduction was significant in both EEMS and methotrexate groups. Biochemical analyses showed elevated markers in the arthritic control group, which were normalized by EEMS and methotrexate. Notably, EEMS (400 mg/kg) significantly reduced cathepsin-D levels compared to the positive control. EEMS administration also lowered hepatic lipid peroxidation and increased endogenous antioxidants (SOD, GSH, and GPX). The 200 and 400 mg/kg doses reduced the iNOS/GADPH ratio, while the 400 mg/kg dose restored cellular and joint structure and significantly decreased IL1 levels.

Conclusions: In conclusion, EEMS demonstrated substantial protective effects, mitigating health risks associated with chronic inflammation such as arthritis. These findings underscore the ethnomedical potential of Myxopyrum serratulum as a promising anti-inflammatory and anti-arthritis agent. The study suggests that EEMS could be a viable alternative or complementary therapy for RA, offering therapeutic benefits with potentially fewer side effects than current treatments.

Myxopyrum serratulum A.W. Hill乙醇提取物的抗炎和抗关节炎活性。
背景:类风湿性关节炎(RA)是一种使人衰弱的炎症性疾病,其特点是免疫系统针对关节过度活跃,导致炎症和剧烈疼痛。虽然目前的类风湿性关节炎疗法能有效缓解症状,但往往会产生严重的副作用。本研究旨在利用动物模型评估 Myxopyrum serratulum A.W. Hill 的乙醇提取物(EEMS)的抗炎和抗关节炎特性:对大鼠进行的 EEMS(2000 毫克/千克,p.o.)急性毒性研究显示,最高剂量无毒性或死亡率。用卡拉胶诱发炎症,然后用不同剂量的 EEMS(100、200 和 400 毫克/千克,口服)和双氯芬酸处理大鼠,以评估抗炎效果。使用完全弗氏佐剂(CFA)诱导的炎症评估了 EEMS 的抗关节炎功效,并将其与甲氨蝶呤进行了比较。结果显示,EEMS具有剂量依赖性抗炎作用,并能逆转治疗组因关节炎引起的体重减轻。EEMS组和甲氨蝶呤组的爪体积均有明显缩小。生化分析表明,关节炎对照组的标志物升高,而 EEMS 和甲氨蝶呤可使其恢复正常。值得注意的是,与阳性对照组相比,EEMS(400 毫克/千克)能显著降低 cathepsin-D 的水平。服用 EEMS 还能降低肝脏脂质过氧化反应,增加内源性抗氧化剂(SOD、GSH 和 GPX)。200和400毫克/千克剂量降低了iNOS/GADPH比率,而400毫克/千克剂量则恢复了细胞和关节结构,并显著降低了IL1水平:总之,EEMS 显示出巨大的保护作用,可减轻与慢性炎症(如关节炎)相关的健康风险。这些发现凸显了丝核菌(Myxopyrum serratulum)作为一种抗炎和抗关节炎药物的民族医药潜力。该研究表明,EEMS可以成为治疗RA的一种可行的替代疗法或辅助疗法,与目前的治疗方法相比,其治疗效果好,副作用可能更小。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.40
自引率
0.00%
发文量
32
审稿时长
8 weeks
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