Detection and Analysis of Florfenicol Residues and Metabolites in Nile Tilapia (Oreochromis niloticus) Tissues Post-Oral Administration in Tropical Waters.

IF 1.5 4区 农林科学 Q3 PHARMACOLOGY & PHARMACY
Anna Paula R Queiroga, Gabriela F P Souza, Jonas Augusto R Paschoal, Airton Gonçalves Salles, Michael Schloter, Inácio Mateus Assane, Fabiana Pilarski, André Tadeu Gotardo, Silvana Lima Górniak, Susanne Rath
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引用次数: 0

Abstract

Water temperature is a critical environmental parameter that significantly influences fish metabolism. This study assessed the metabolism of florfenicol (FF) in tilapia (Oreochromis niloticus) at water temperatures typical of tropical and subtropical regions. Fish were treated with FF by oral administration of a dose of 10 mg kg-1 bw for 10 consecutive days. Fish fillet, liver, and kidney were sampled during the treatment phase (1, 5, and 10 days) and posttreatment (1, 2, 3, and 5 days after the last FF administration). FF, florfenicol amine (FFA), monochloro florfenicol (FFCl), and florfenicol alcohol (FFOH) were determined in the sampled tissues using a validated LC-LC-MS/MS method. The highest FF, FFA, and FFOH concentrations were determined on day 5 during the treatment phase. For FF, the concentration order is kidney > liver > fillet, while for the metabolites FFOH and FFA, the order is liver > kidney > fillet. In fillet and liver, the concentrations of FFOH were higher than the FFA concentrations, indicating that FFOH was the primary metabolite in these tissues. FFCl was only quantified at concentrations lower than 90 μg kg-1 in all tissues. The results indicated that FF can be readily absorbed and rapidly eliminated in tilapia cultivated in warm water environments. This study revealed FFOH as the primary and most persistent metabolite in tilapia farmed in warm water, followed by FFA.

热带水域口服氟苯尼考后尼罗罗非鱼(Oreochromis niloticus)组织中氟苯尼考残留和代谢物的检测与分析。
水温是对鱼类新陈代谢有重要影响的关键环境参数。本研究评估了罗非鱼(Oreochromis niloticus)在热带和亚热带典型水温条件下的氟苯尼考(FF)代谢情况。鱼类连续 10 天口服剂量为 10 毫克/千克-1 体重的氟苯尼考(FF)。在处理阶段(1、5 和 10 天)和处理后(最后一次给药后的 1、2、3 和 5 天)对鱼片、肝脏和肾脏进行采样。采用经过验证的 LC-LC-MS/MS 方法测定了取样组织中的 FF、氟苯尼考胺(FFA)、单氯氟苯尼考(FFCl)和氟苯尼考醇(FFOH)。在治疗阶段的第 5 天,FF、FFA 和 FFOH 的浓度最高。FF的浓度顺序为肾脏>肝脏>鱼片,而代谢物FFOH和FFA的浓度顺序为肝脏>肾脏>鱼片。在鱼片和肝脏中,FFOH 的浓度高于 FFA 的浓度,表明 FFOH 是这些组织中的主要代谢物。在所有组织中,只有当 FFCl 的浓度低于 90 μg kg-1 时才会被定量。研究结果表明,在温水环境中养殖的罗非鱼很容易吸收并快速消除 FF。这项研究表明,FFOH 是温水养殖罗非鱼体内最主要、最持久的代谢物,其次是 FFA。
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来源期刊
CiteScore
3.10
自引率
15.40%
发文量
69
审稿时长
8-16 weeks
期刊介绍: The Journal of Veterinary Pharmacology and Therapeutics (JVPT) is an international journal devoted to the publication of scientific papers in the basic and clinical aspects of veterinary pharmacology and toxicology, whether the study is in vitro, in vivo, ex vivo or in silico. The Journal is a forum for recent scientific information and developments in the discipline of veterinary pharmacology, including toxicology and therapeutics. Studies that are entirely in vitro will not be considered within the scope of JVPT unless the study has direct relevance to the use of the drug (including toxicants and feed additives) in veterinary species, or that it can be clearly demonstrated that a similar outcome would be expected in vivo. These studies should consider approved or widely used veterinary drugs and/or drugs with broad applicability to veterinary species.
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