Antimicrobial resistance characterization of Enterococcus faecium, Enterococcus faecalis and Enterococcus hirae isolated from marine coastal recreational waters in the State of São Paulo, Brazil.
Giovanna Silva Santiago, Milena Dropa, Solange Martone-Rocha, Talita Pereira Dos Santos, Vasco Tulio de Moura Gomes, Mikaela Renata Funada Barbosa, Maria Tereza Pepe Razzolini
{"title":"Antimicrobial resistance characterization of <i>Enterococcus faecium, Enterococcus faecalis</i> and <i>Enterococcus hirae</i> isolated from marine coastal recreational waters in the State of São Paulo, Brazil.","authors":"Giovanna Silva Santiago, Milena Dropa, Solange Martone-Rocha, Talita Pereira Dos Santos, Vasco Tulio de Moura Gomes, Mikaela Renata Funada Barbosa, Maria Tereza Pepe Razzolini","doi":"10.2166/wh.2024.098","DOIUrl":null,"url":null,"abstract":"<p><p>Coastal water quality is facing increasing threats due to human activities. Their contamination by sewage discharges poses significant risks to the environment and public health. We aimed to investigate the presence of antibiotic-resistant <i>Enterococcus</i> in beach waters. Over a 10-month period, samples were collected from four beaches in the State of São Paulo (Brazil). <i>Enterococcus</i> isolates underwent matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) and molecular analysis for accurate genus and species identification. The antimicrobial susceptibility for 14 antibiotics was evaluated using the disc diffusion method followed by a multidrug-resistance (MDR) classification. PCR amplification method was used to detect antimicrobial resistance genes (ARGs). Our findings revealed the prevalence of <i>Enterococcus faecalis</i>, <i>E. faecium</i> and <i>E. hirae</i>. Out of 130 isolates, 118 were resistant to multiple antibiotics. The detection of resistance genes provided evidence of the potential transfer of antibiotic resistance within the environment. Our findings underscore the necessity for continuous research and surveillance to enhance understanding of the pathogenicity and antimicrobial resistance mechanisms of <i>Enterococcus</i>, which is crucial to implement effective measures to preserve the integrity of coastal ecosystems.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of water and health","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.2166/wh.2024.098","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/23 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Coastal water quality is facing increasing threats due to human activities. Their contamination by sewage discharges poses significant risks to the environment and public health. We aimed to investigate the presence of antibiotic-resistant Enterococcus in beach waters. Over a 10-month period, samples were collected from four beaches in the State of São Paulo (Brazil). Enterococcus isolates underwent matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) and molecular analysis for accurate genus and species identification. The antimicrobial susceptibility for 14 antibiotics was evaluated using the disc diffusion method followed by a multidrug-resistance (MDR) classification. PCR amplification method was used to detect antimicrobial resistance genes (ARGs). Our findings revealed the prevalence of Enterococcus faecalis, E. faecium and E. hirae. Out of 130 isolates, 118 were resistant to multiple antibiotics. The detection of resistance genes provided evidence of the potential transfer of antibiotic resistance within the environment. Our findings underscore the necessity for continuous research and surveillance to enhance understanding of the pathogenicity and antimicrobial resistance mechanisms of Enterococcus, which is crucial to implement effective measures to preserve the integrity of coastal ecosystems.
期刊介绍:
Journal of Water and Health is a peer-reviewed journal devoted to the dissemination of information on the health implications and control of waterborne microorganisms and chemical substances in the broadest sense for developing and developed countries worldwide. This is to include microbial toxins, chemical quality and the aesthetic qualities of water.