Antimicrobial resistance characterization of Enterococcus faecium, Enterococcus faecalis and Enterococcus hirae isolated from marine coastal recreational waters in the State of São Paulo, Brazil.

IF 2.5 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES
Journal of water and health Pub Date : 2024-09-01 Epub Date: 2024-08-23 DOI:10.2166/wh.2024.098
Giovanna Silva Santiago, Milena Dropa, Solange Martone-Rocha, Talita Pereira Dos Santos, Vasco Tulio de Moura Gomes, Mikaela Renata Funada Barbosa, Maria Tereza Pepe Razzolini
{"title":"Antimicrobial resistance characterization of <i>Enterococcus faecium, Enterococcus faecalis</i> and <i>Enterococcus hirae</i> isolated from marine coastal recreational waters in the State of São Paulo, Brazil.","authors":"Giovanna Silva Santiago, Milena Dropa, Solange Martone-Rocha, Talita Pereira Dos Santos, Vasco Tulio de Moura Gomes, Mikaela Renata Funada Barbosa, Maria Tereza Pepe Razzolini","doi":"10.2166/wh.2024.098","DOIUrl":null,"url":null,"abstract":"<p><p>Coastal water quality is facing increasing threats due to human activities. Their contamination by sewage discharges poses significant risks to the environment and public health. We aimed to investigate the presence of antibiotic-resistant <i>Enterococcus</i> in beach waters. Over a 10-month period, samples were collected from four beaches in the State of São Paulo (Brazil). <i>Enterococcus</i> isolates underwent matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) and molecular analysis for accurate genus and species identification. The antimicrobial susceptibility for 14 antibiotics was evaluated using the disc diffusion method followed by a multidrug-resistance (MDR) classification. PCR amplification method was used to detect antimicrobial resistance genes (ARGs). Our findings revealed the prevalence of <i>Enterococcus faecalis</i>, <i>E. faecium</i> and <i>E. hirae</i>. Out of 130 isolates, 118 were resistant to multiple antibiotics. The detection of resistance genes provided evidence of the potential transfer of antibiotic resistance within the environment. Our findings underscore the necessity for continuous research and surveillance to enhance understanding of the pathogenicity and antimicrobial resistance mechanisms of <i>Enterococcus</i>, which is crucial to implement effective measures to preserve the integrity of coastal ecosystems.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of water and health","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.2166/wh.2024.098","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/23 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Coastal water quality is facing increasing threats due to human activities. Their contamination by sewage discharges poses significant risks to the environment and public health. We aimed to investigate the presence of antibiotic-resistant Enterococcus in beach waters. Over a 10-month period, samples were collected from four beaches in the State of São Paulo (Brazil). Enterococcus isolates underwent matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) and molecular analysis for accurate genus and species identification. The antimicrobial susceptibility for 14 antibiotics was evaluated using the disc diffusion method followed by a multidrug-resistance (MDR) classification. PCR amplification method was used to detect antimicrobial resistance genes (ARGs). Our findings revealed the prevalence of Enterococcus faecalis, E. faecium and E. hirae. Out of 130 isolates, 118 were resistant to multiple antibiotics. The detection of resistance genes provided evidence of the potential transfer of antibiotic resistance within the environment. Our findings underscore the necessity for continuous research and surveillance to enhance understanding of the pathogenicity and antimicrobial resistance mechanisms of Enterococcus, which is crucial to implement effective measures to preserve the integrity of coastal ecosystems.

从巴西圣保罗州海滨休闲水域分离的粪肠球菌、粪肠球菌和平肠球菌的抗菌药耐药性特征。
由于人类活动的影响,沿海水质正面临着越来越大的威胁。污水排放对水质造成的污染对环境和公众健康构成了重大威胁。我们的目的是调查海滩水域中是否存在抗生素耐药肠球菌。在 10 个月的时间里,我们从圣保罗州(巴西)的四个海滩采集了样本。对分离出的肠球菌进行了基质辅助激光解吸电离飞行时间质谱分析(MALDI-TOF/MS)和分子分析,以准确鉴定菌属和菌种。采用盘扩散法评估了 14 种抗生素的抗菌敏感性,然后进行了多重耐药性(MDR)分类。PCR 扩增法用于检测抗菌药耐药基因(ARGs)。我们的研究结果表明,粪肠球菌、粪大肠杆菌和平肠球菌普遍存在。在 130 个分离株中,118 个对多种抗生素具有耐药性。耐药基因的检测提供了环境中抗生素耐药性潜在转移的证据。我们的研究结果表明,有必要继续开展研究和监测,以加深对肠球菌致病性和抗菌药耐药性机制的了解,这对于采取有效措施保护沿海生态系统的完整性至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of water and health
Journal of water and health 环境科学-环境科学
CiteScore
3.60
自引率
8.70%
发文量
110
审稿时长
18-36 weeks
期刊介绍: Journal of Water and Health is a peer-reviewed journal devoted to the dissemination of information on the health implications and control of waterborne microorganisms and chemical substances in the broadest sense for developing and developed countries worldwide. This is to include microbial toxins, chemical quality and the aesthetic qualities of water.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信