Protective effect of afamin protein against oxidative stress related injury in human ovarian granulosa cells.

IF 3.8 3区医学 Q1 REPRODUCTIVE BIOLOGY

Journal of Ovarian Research Pub Date : 2024-09-28 DOI:10.1186/s13048-024-01511-3

Qiang Zhang, Xiaoyu Zheng, Xueying Zhang, Lianwen Zheng

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引用次数: 0

Abstract

Background: Ovarian granulosa cells (GCs) play crucial roles in follicular growth and development. Their normal function is influenced by various factors, including oxidative stress, which is a significant factor. Afamin protein is a vitamin E-specific binding protein that acts as a vitamin E carrier in follicular fluid. Although the mechanism of the protective effect of afamin on human ovarian GCs is still unclear, there is evidence it has an antioxidant effect in neuronal cells.

Methods: In this study, we investigated the protective effects of afamin proteins on testosterone propionate (TP)-induced ovarian GCs using a human ovarian tumor granulosa cell line (KGN).

Results: The results showed that afamin reduced TP-induced oxidative stress in KGN cells by decreasing the levels of oxidative damage markers, enhancing the activity of antioxidant enzymes, and exerting a protective effect on GCs. Supplementation with afamin repaired mitochondrial dysfunction by improving mitochondrial membrane potential damage and ATP levels. It counteracted TP-induced apoptosis by decreasing the activity of Caspase-3 and upregulating the expression of the anti-apoptotic gene (BCL-2) while downregulating the expression of the pro-apoptotic gene BCL-2-associated X protein (BAX). In addition, afamin regulated the expression of genes related to ovarian steroid hormone synthesis, ameliorating the endocrine dysfunction observed in TP-induced KGN cells.

Conclusion: Therefore, Afamin proteins may serve as important complementary factors that protect GCs from other types of damage, such as oxidative stress, and may help improve ovarian follicle quality and female reproductive function.

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阿法明蛋白对人卵巢颗粒细胞氧化应激相关损伤的保护作用

背景：卵巢颗粒细胞（GCs）在卵泡的生长和发育中起着至关重要的作用。它们的正常功能受到各种因素的影响，其中氧化应激是一个重要因素。Afamin蛋白是一种维生素E特异性结合蛋白，在卵泡液中充当维生素E载体。虽然阿法明对人类卵巢GCs的保护作用机制尚不清楚，但有证据表明它对神经元细胞有抗氧化作用：本研究利用人体卵巢肿瘤颗粒细胞系（KGN）研究了阿法明蛋白对丙酸睾酮（TP）诱导的卵巢GCs的保护作用：结果表明，阿法明通过降低氧化损伤标志物的水平、提高抗氧化酶的活性，减少了TP诱导的KGN细胞氧化应激，对GCs具有保护作用。通过改善线粒体膜电位损伤和 ATP 水平，补充阿法明可修复线粒体功能障碍。它通过降低 Caspase-3 的活性，上调抗凋亡基因（BCL-2）的表达，同时下调促凋亡基因 BCL-2-associated X 蛋白（BAX）的表达，从而对抗 TP 诱导的细胞凋亡。此外，阿法明还能调节卵巢类固醇激素合成相关基因的表达，改善TP诱导的KGN细胞的内分泌功能障碍：因此，阿法明蛋白可能是保护GC免受氧化应激等其他类型损伤的重要补充因子，有助于改善卵泡质量和女性生殖功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Ovarian Research REPRODUCTIVE BIOLOGY-

CiteScore

6.20

自引率

2.50%

发文量

125

审稿时长

>12 weeks

期刊介绍： Journal of Ovarian Research is an open access, peer reviewed, online journal that aims to provide a forum for high-quality basic and clinical research on ovarian function, abnormalities, and cancer. The journal focuses on research that provides new insights into ovarian functions as well as prevention and treatment of diseases afflicting the organ. Topical areas include, but are not restricted to: Ovary development, hormone secretion and regulation Follicle growth and ovulation Infertility and Polycystic ovarian syndrome Regulation of pituitary and other biological functions by ovarian hormones Ovarian cancer, its prevention, diagnosis and treatment Drug development and screening Role of stem cells in ovary development and function.