Evaluation and Molecular Characterization of Colistin-Resistant Isolates of Pseudomonas aeruginosa and Klebsiella pneumoniae from the Infected Wounds of Hospitalized Patients.

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Journal of microbiology and biotechnology Pub Date : 2024-10-28 Epub Date: 2024-08-16 DOI:10.4014/jmb.2407.07005
Munaza Ijaz, Madiha Khan, Haya Yasin
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引用次数: 0

Abstract

This study was planned to determine the colistin-resistant (CR) gene distribution among two species of gram-negative bacteria, Pseudomonas aeruginosa and Klebsiella pneumoniae. In total, 50 isolates of K. pneumoniae (14 isolates, 28%) and P. aeruginosa (36 isolates, 72%) were isolated between August 2023 and October 2023 from clinical wound samples at Jinnah Hospital and Lahore General Hospital Lahore, Pakistan. To determine the resistance genes linked to CR and assess antimicrobial susceptibility, all isolates were kept at -80°C in 15% glycerol broth. Using the right primer sets, a polymerase chain reaction (PCR) was utilized to identify the CR-associated mcr-1 gene of the gram-negative isolates. Out of 50, 40 isolates (80%) showed resistance against colistin with MICs of 8 and 128 μg/ml. The majority (97%) of P. aeruginosa CR strains were considered multidrug resistant (MDR). All K. pneumoniae isolates were resistant to cefepime, cotrimoxazole, ceftriaxone, and imipenem. The clinical CR isolates of P. aeruginosa were highly resistant to ceftriaxone, imipenem, cefepime, cotrimoxazole, ciprofloxacin, amikacin, and piperacillin/tazobactum. The antibiotic resistance pattern was terrifyingly high among both bacterial species. According to the PCR results, CR was prevalent among the gram-negative samples, and the mcr-1 gene was positive in 6/40 (15%) of the CR isolates, including four P. aeruginosa and two K. pneumoniae strains. The high CR (80%) reported in this research is cause for concern and underscores an urgent need to use colistin in a limited and logical manner, similar to other antibiotics.

对住院患者感染伤口中的铜绿假单胞菌和肺炎克雷伯菌耐考利司汀分离物进行评估和分子特征描述。
本研究计划确定两种革兰氏阴性细菌(铜绿假单胞菌和肺炎克雷伯菌)的耐可乐定(CR)基因分布情况。2023 年 8 月至 2023 年 10 月期间,从巴基斯坦拉合尔真纳医院和拉合尔总医院的临床伤口样本中共分离出 50 株肺炎克雷伯菌(14 株,占 28%)和铜绿假单胞菌(36 株,占 72%)。为确定与 CR 相关的耐药基因并评估抗菌药敏感性,所有分离物均保存在 -80°C 的 15% 甘油肉汤中。使用正确的引物组,利用聚合酶链反应(PCR)来确定革兰氏阴性分离株的 CR 相关 mcr-1 基因。在 50 个分离株中,有 40 个(80%)对可乐定产生耐药性,耐药浓度分别为 8 和 128 μg/ml。大多数(97%)铜绿假单胞菌 CR 株系被认为具有多重耐药性(MDR)。所有肺炎克雷伯菌株均对头孢吡肟、复方新诺明、头孢曲松和亚胺培南耐药。铜绿假单胞菌的临床 CR 分离物对头孢曲松、亚胺培南、头孢吡肟、复方新诺明、环丙沙星、阿米卡星和哌拉西林/他唑巴坦高度耐药。两种细菌对抗生素的耐药性都非常高。根据聚合酶链式反应(PCR)结果,革兰氏阴性样本中普遍存在耐药菌株,6/40(15%)的耐药菌株中 mcr-1 基因呈阳性,其中包括 4 株铜绿假单胞菌和 2 株肺炎双球菌。这项研究中报告的高 CR(80%)令人担忧,并强调了以有限和合理的方式使用可乐定(类似于其他抗生素)的迫切需要。
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来源期刊
Journal of microbiology and biotechnology
Journal of microbiology and biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
5.50
自引率
3.60%
发文量
151
审稿时长
2 months
期刊介绍: The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.
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