Sajin Ponnan, D Narayana Rao, Sri Ram G Naraharisetty
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引用次数: 0
Abstract
This work investigated the photoexcitation and relaxation kinetics of the ADS800AT dye dissolved in different solvents using transient absorption spectroscopy (TAS) with a white-light continuum probe. The dye was dissolved in various solvents, including dichloromethane (DCM), 1,2-dichlorobenzene (DCB), ethanol, and methanol, to study their impact on the dye's characteristics. The linear absorption peak varied from 835 to 809 nm, depending on the polarity of the solvent, and the pump wavelength for TAS was chosen accordingly. We observed ground-state bleaching and excited-state absorption after exciting the dye with the pump pulse. Global analysis was performed using Glotaran software to fit exponential decay curve models, allowing us to determine the relaxation time of the excited molecule. The relaxation time varied from 198 ps to 508 ps across the different solvents, decreasing as the polarity of the solvent increased. Additionally, we could experimentally correlate the dye molecule's nonlinear properties with the solvent's polarity.
期刊介绍：
Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
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