Thermophile-fermented feed modulates the gut microbiota related to lactate metabolism in pigs.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Applied Microbiology Pub Date : 2024-10-03 DOI:10.1093/jambio/lxae254

Shota Yoshikawa, Kaede Itaya, Ryo Hoshina, Yukihiro Tashiro, Wataru Suda, Yuichiro Cho, Makiko Matsuura, Chie Shindo, Toshiyuki Ito, Masahira Hattori, Hirokuni Miyamoto, Hiroaki Kodama

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引用次数: 0

Abstract

Aims: Extracts of fermented feed obtained via fermentation of marine animal resources with thermophilic Bacillaceae bacteria increase the fecundity of livestock. The intestinal bacterial profiles in response to long-term administration of this extract to pigs were investigated.

Methods and results: Half of a swine farm was supplied with potable water containing an extract of fermented feed for more than 2 years, whereas the other half was supplied with potable water without the extract. Feces from 6-month-old pigs rearing in these two areas were collected. 16S rRNA gene sequencing and isolation of lactic acid bacteria revealed an increase in the D/L-lactate-producing bacterium, Lactobacillus amylovorus, and a decrease in several members of Clostridiales following administration of fermented feed. A lactate-utilizing bacterium, Megasphaera elsdenii, was more abundant in the feces of pigs in the fermented feed group. All representative isolates of M. elsdenii showed rapid utilization of D-lactate relative to L-lactate, and butyrate and valerate were the main products.

Conclusion: The probiotic effect of fermented feed is associated with the modulation of lactate metabolism in the digestive organs of pigs.

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嗜热菌发酵饲料可调节与猪乳酸代谢有关的肠道微生物群。

目的：通过嗜热芽孢杆菌发酵海洋动物资源获得的发酵饲料提取物可提高家畜的繁殖力。我们对猪长期服用这种提取物后的肠道细菌情况进行了调查：在一个猪场中，一半猪场供应含有发酵饲料提取物的饮用水，为期两年多；另一半猪场供应不含提取物的饮用水。收集了在这两个地区饲养的六个月大的猪的粪便。16S rRNA 基因测序和乳酸菌分离结果显示，使用发酵饲料后，D/L-乳酸菌（淀粉乳杆菌）增加，而梭状芽孢杆菌（Clostridiales）的几种成员减少。发酵饲料组猪粪便中的乳酸利用细菌 Megasphaera elsdenii 数量更多。所有具有代表性的 M. elsdenii 分离物都显示，相对于 L-乳酸盐，D-乳酸盐的利用速度更快，丁酸盐和戊酸盐是主要产物：结论：发酵饲料的益生作用与猪消化器官的乳酸代谢调节有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.