Characterization and long-read sequencing of biofilms formed by the microbiota present on inert surfaces in poultry slaughterhouses

IF 5 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
Sarah Panera-Martínez , Cristina Rodríguez-Melcón , Félix Riesco-Peláez , Daniel Rodríguez-Campos , Carlos Alonso-Calleja , Rosa Capita
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Abstract

Cross-contamination from inert slaughterhouse surfaces is among the main sources of contamination of poultry. The objective of the research reported here was to characterize the biofilms formed by the microbiota present on various surfaces in two poultry slaughterhouses in north-western Spain. Forty-four samples (22 from each slaughterhouse) were taken by swab rubbing at different points along the processing line (from stunning to cutting). The microbiota on all surfaces was able to form biofilms, which were studied by scanning confocal laser microscopy. The total biovolume in the observation field of 16,078.24 μm2 ranged from 22,106.8 ± 5544.3 μm3 to 414,229.6 ± 1621.0 μm3. Average values were higher in abattoir A than in abattoir B, with significant differences (P < 0.05) between surfaces. The percentage of biovolume of Gram-positive bacteria ranged between 0.02 % and 5.38 %. The highest percentages of Gram-positive bacteria were detected towards the beginning of the processing line. The microbiota of the biofilms was identified using long-read sequencing techniques (Oxford Nanopore). The predominant genera (found in >50.0 % of the biofilms) were Pseudomonas, Citrobacter, Klebsiella, Serratia, Escherichia, Enterobacter, Stenotrophomonas, Salmonella, Shewanella, Acinetobacter and Aeromonas. In addition, some pathogenic bacteria were detected, including Salmonella (31 surfaces), Yersinia enterocolitica (12), Escherichia coli O157:H7 (6), Campylobacter spp. (4) and Listeria monocytogenes (3). This research work has permitted identification of the most contaminated surfaces in poultry abattoirs and can serve as a starting point for the design of more effective cleaning and disinfection protocols.

Abstract Image

对家禽屠宰场惰性表面微生物群形成的生物膜进行特征描述和长读测序。
屠宰场惰性表面的交叉污染是家禽污染的主要来源之一。本文报告的研究目的是描述西班牙西北部两个家禽屠宰场不同表面微生物群形成的生物膜的特征。研究人员在加工生产线的不同位置(从宰杀到切割)用棉签擦拭的方式采集了 44 份样本(每个屠宰场 22 份)。所有表面上的微生物群都能形成生物膜,并通过扫描共焦激光显微镜对其进行了研究。观察区域的总生物体积为 16,078.24 μm2 ,范围从 22,106.8 ± 5544.3 μm3 到 414,229.6 ± 1621.0 μm3。屠宰场 A 的平均值高于屠宰场 B,生物膜中差异显著(P 50.0 %)的细菌有假单胞菌、柠檬酸杆菌、克雷伯氏菌、沙雷氏菌、大肠埃希氏菌、肠杆菌、血单胞菌、沙门氏菌、雪旺菌、醋氨单胞菌和气单胞菌。此外,还检测到一些致病菌,包括沙门氏菌(31 个表面)、小肠结肠耶尔森菌(12 个)、大肠杆菌 O157:H7 (6 个)、弯曲杆菌属(4 个)和单核细胞增生李斯特菌(3 个)。这项研究工作有助于确定家禽屠宰场中受污染最严重的表面,可作为设计更有效的清洁和消毒方案的起点。
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来源期刊
International journal of food microbiology
International journal of food microbiology 工程技术-食品科技
CiteScore
10.40
自引率
5.60%
发文量
322
审稿时长
65 days
期刊介绍: The International Journal of Food Microbiology publishes papers dealing with all aspects of food microbiology. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. They should provide scientific or technological advancement in the specific field of interest of the journal and enhance its strong international reputation. Preliminary or confirmatory results as well as contributions not strictly related to food microbiology will not be considered for publication.
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