Antibiotic-Loaded Dendrimer Hydrogels in Periodontal Bone Regeneration: An In Vitro Release Feasibility Study.

IF 5 3区 化学 Q1 POLYMER SCIENCE
Gels Pub Date : 2024-09-14 DOI:10.3390/gels10090593
Nicholas Yesbeck, Da Huang, Caroline Carrico, Parthasarathy Madurantakam, Hu Yang
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引用次数: 0

Abstract

The prescription of a course of oral antibiotics following bone grafting procedures is a common practice in clinical periodontics to reduce surgical site infections. The goal of this study is to characterize the release profile of antibiotics via local delivery using dendrimer hydrogels (DH) and to analyze the effect of two different particulate bone allografts on the release of the antibiotics in vitro. DH were synthesized from polyamidoamine (PAMAM) dendrimer G5 and polyethylene glycol diacrylate, and cefazolin was chosen as the antibiotic. The antibiotic-loaded samples were bathed in PBS and incubated at 37 °C; aliquots were taken (1 h, 2 h, 3 h, 4 h, 5 h, 6 h, 12 h, 24 h, 48 h, 72 h) and analyzed using HPLC to determine the amounts of released cefazolin. In samples with DH, the estimated maximum concentration of cefazolin was 36.97 ± 2.39 μg/mL (95% CI: 34.58-39.36) with 50% released in 4.17 h (95%: 3.26-5.07) and an estimated growth rate of 0.27 (95% CI: 0.17-0.37). For samples without DH, the estimated maximum concentration of cefazolin was 167.4 ± 7.0 μg/mL (95% CI: 160.4-174.4) with 50% released in 2.36 h (95% CI: 2.05-2.67) and an estimated growth rate of 0.70 (95% CI: 0.54-0.87). We conclude that DH are a promising platform for sustained antibiotic release and that the presence of bone grafts did not significantly affect their release.

抗生素负载树枝状聚合物水凝胶在牙周骨再生中的应用:体外释放可行性研究。
为减少手术部位感染,临床牙周病学常用的做法是在植骨手术后处方一个疗程的口服抗生素。本研究的目的是通过使用树枝状聚合物水凝胶(DH)进行局部给药来描述抗生素的释放特征,并分析两种不同颗粒骨异体移植对抗生素体外释放的影响。DH 由聚氨基胺(PAMAM)树枝状聚合物 G5 和聚乙二醇二丙烯酸酯合成,抗生素选择头孢唑啉。将负载抗生素的样品浸泡在 PBS 中并在 37 ℃ 温育;取等分样品(1 小时、2 小时、3 小时、4 小时、5 小时、6 小时、12 小时、24 小时、48 小时、72 小时)并使用 HPLC 进行分析,以确定头孢唑啉的释放量。在有 DH 的样品中,头孢唑啉的最大浓度估计为 36.97 ± 2.39 μg/mL(95% CI:34.58-39.36),50%的释放时间为 4.17 小时(95%:3.26-5.07),增长率估计为 0.27(95% CI:0.17-0.37)。对于不含 DH 的样本,头孢唑啉的估计最大浓度为 167.4 ± 7.0 μg/mL(95% CI:160.4-174.4),50% 的释放时间为 2.36 小时(95% CI:2.05-2.67),估计增长率为 0.70(95% CI:0.54-0.87)。我们的结论是,DH 是一种很有前景的持续释放抗生素的平台,骨移植的存在对其释放没有明显影响。
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来源期刊
Gels
Gels POLYMER SCIENCE-
CiteScore
4.70
自引率
19.60%
发文量
707
审稿时长
11 weeks
期刊介绍: The journal Gels (ISSN 2310-2861) is an international, open access journal on physical (supramolecular) and chemical gel-based materials. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the maximum length of the papers, and full experimental details must be provided so that the results can be reproduced. Short communications, full research papers and review papers are accepted formats for the preparation of the manuscripts. Gels aims to serve as a reference journal with a focus on gel materials for researchers working in both academia and industry. Therefore, papers demonstrating practical applications of these materials are particularly welcome. Occasionally, invited contributions (i.e., original research and review articles) on emerging issues and high-tech applications of gels are published as special issues.
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