Enhancing Dental Alginate with Syzygium aromaticum, Zingiber officinale and Green Silver Nanoparticles: A Nature-Enhanced Approach for Superior Infection Control.

IF 5 3区 化学 Q1 POLYMER SCIENCE
Gels Pub Date : 2024-09-20 DOI:10.3390/gels10090600
Lamia Singer, Leonie Beuter, Sabina Karacic, Gabriele Bierbaum, Jesenko Karacic, Christoph Bourauel
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引用次数: 0

Abstract

Objectives: The study addresses the challenge of cross-infection in dentistry, focusing on improving disinfection protocols for dental hydrocolloid gel materials. This research aimed to incorporate chlorhexidine, natural plant extracts, and green-synthesized silver nanoparticles (AgNPs) into dental alginate to enhance safety and hygiene standards in dental practices.

Methods: Conventional dental alginate served as the control, with experimental groups including alginate modified with 0.2% chlorhexidine (CHX-group) and alginate incorporating AgNPs synthesized from Syzygium aromaticum (SA) and Zingiber officinale (ZO) extracts (SA + AgNPs and ZO + AgNPs groups). Plant extracts were analyzed via GC/MS to confirm their composition. UV-visible spectroscopy, SEM, and EDX characterized the synthesized AgNPs. Antimicrobial efficacy against Candida albicans, Streptococcus mutans, and methicillin-resistant and methicillin-sensitive Staphylococcus aureus was evaluated using agar well diffusion assays. The dimensional accuracy of alginate impressions was assessed according to ISO 21563:2021 standards.

Results: Chemical analysis of SA and ZO extracts identified 60 and 43 active compounds, respectively, supporting their use in AgNP synthesis. UV-visible spectroscopy, SEM, and EDX confirmed the formation of spherical AgNPs using SA and ZO extracts. Modified groups showed inhibitory activity against Streptococcus mutans and methicillin-resistant (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA), in contrast to the unmodified control. Both AgNP-modified groups demonstrated efficacy comparable to the CHX-group against MRSA and MSSA, with SA + AgNP showing superior performance against C. albicans. The dimensional accuracy of all groups was within clinically acceptable ranges as reported in the literature (0.027-0.083 mm).

Discussion: CHX, silver nitrate, and green-synthesized AgNPs present promising options for developing self-disinfecting alginate impression materials. Utilizing plant extracts in AgNP synthesis offers a safe, efficient, and synergistic approach between metal ions and phytotherapeutic agents. This approach could potentially enhance disinfection efficacy without compromising material performance, improving dental safety and hygiene.

用芳香茜草、细辛和绿色银纳米粒子增强藻酸盐牙膏:自然强化法实现卓越的感染控制。
研究目的本研究针对牙科中交叉感染的挑战,重点是改进牙科水胶体凝胶材料的消毒方案。本研究旨在将洗必泰、天然植物提取物和绿色合成的银纳米粒子(AgNPs)加入牙科海藻酸盐中,以提高牙科实践中的安全和卫生标准:传统的牙科海藻酸盐作为对照组,实验组包括用 0.2% 洗必泰改性的海藻酸盐(CHX 组),以及加入由芳香茜草(SA)和欧当归(ZO)提取物合成的 AgNPs 的海藻酸盐(SA + AgNPs 组和 ZO + AgNPs 组)。植物提取物通过气相色谱/质谱仪进行分析,以确认其成分。紫外-可见光谱、扫描电子显微镜和电离辐射显微镜对合成的 AgNPs 进行了表征。使用琼脂井扩散试验评估了对白色念珠菌、变异链球菌、耐甲氧西林和对甲氧西林敏感的金黄色葡萄球菌的抗菌效果。根据 ISO 21563:2021 标准评估了藻酸盐印模的尺寸精度:结果:SA 和 ZO 提取物的化学分析分别鉴定出 60 和 43 种活性化合物,支持它们在 AgNP 合成中的应用。紫外-可见光谱、扫描电子显微镜和电离氧化物显微镜证实了使用 SA 和 ZO 提取物形成的球形 AgNPs。与未修饰的对照组相比,修饰组对变异链球菌、耐甲氧西林(MRSA)和甲氧西林敏感金黄色葡萄球菌(MSSA)具有抑制活性。AgNP 改性组对 MRSA 和 MSSA 的疗效与 CHX 组相当,而 SA + AgNP 组对白葡萄球菌的疗效则更胜一筹。所有组的尺寸精度都在文献报道的临床可接受范围内(0.027-0.083 毫米):讨论:CHX、硝酸银和绿色合成的 AgNPs 为开发自消毒藻酸盐印模材料提供了很好的选择。在 AgNP 合成过程中利用植物提取物提供了一种安全、高效、金属离子与植物治疗剂协同作用的方法。这种方法有可能在不影响材料性能的情况下提高消毒效果,从而改善牙科安全和卫生状况。
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来源期刊
Gels
Gels POLYMER SCIENCE-
CiteScore
4.70
自引率
19.60%
发文量
707
审稿时长
11 weeks
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