Diminished light sensitivities of ON alpha retinal ganglion cells observed in a mouse model of hyperglycemia

IF 3 2区医学 Q1 OPHTHALMOLOGY

Experimental eye research Pub Date : 2024-09-28 DOI:10.1016/j.exer.2024.110113

Qin Wang , Chunghim So , Chunting Qiu , Ting Zhang , Kangyi Yang , Feng Pan

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Abstract

This study aimed to investigate potential functional changes in retinal ganglion cells (RGCs) in a mouse model of hyperglycemia and explore possible therapeutic approaches. Hyperglycemia resembling type 1 diabetes mellitus (DM) was induced in C57BL/6 mice through intraperitoneal injection of streptozotocin (STZ). Blood glucose levels were confirmed to be elevated after 1 week and 4 weeks of injection. Mice with blood glucose levels above 350 mg/mL after 4 weeks of one-dose STZ injection were considered hyperglycemic. The light sensitivity of ON alpha (α) retinal ganglion cells (RGCs), not OFF αRGCs, was reduced in the hyperglycemic mouse model. The number of apoptotic cells, RGCs, and amacrine cells (ACs) remained unaffected at this stage. Similarly, the eletroretinogram (ERG) and optokinetic test results showed no significant differences. The application of picrotoxin (PTX) to block GABA receptors could increase the light sensitivity of ON αRGCs by 1 log unit in hyperglycemic mice. The results show that ON αRGCs may be more susceptible to microenvironmental changes caused by hyperglycemia than OFF αRGCs. This decline in light sensitivity may occur before cell apoptosis during the early stages of the hyperglycemic mouse model but has the potential to be reversed.

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在高血糖小鼠模型中观察到 ON Alpha 视网膜神经节细胞的光敏性减弱

本研究旨在调查高血糖小鼠模型中视网膜神经节细胞（RGC）的潜在功能变化，并探索可能的治疗方法。通过腹腔注射链脲佐菌素（STZ）诱导C57BL/6小鼠出现类似1型糖尿病（DM）的高血糖。注射 1 周和 4 周后证实血糖水平升高。注射一剂 STZ 4 周后血糖水平超过 350 毫克/毫升的小鼠被视为高血糖。在高血糖小鼠模型中，ON α（α）视网膜神经节细胞（RGC）的光敏感性降低，而OFF αRGC的光敏感性没有降低。在这一阶段，凋亡细胞、RGC 和 AC 的数量仍然不受影响。同样，ERG和视神经运动测试结果也无明显差异。应用微毒素（PTX）阻断GABA受体可使高血糖小鼠ON αRGCs的光敏感性提高1个对数单位。结果表明，ON αRGCs可能比OFF αRGCs更容易受到高血糖引起的微环境变化的影响。在高血糖小鼠模型的早期阶段，这种光敏感性的下降可能发生在细胞凋亡之前，但有可能被逆转。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Experimental eye research 医学-眼科学

CiteScore

6.80

自引率

5.90%

发文量

323

审稿时长

66 days

期刊介绍： The primary goal of Experimental Eye Research is to publish original research papers on all aspects of experimental biology of the eye and ocular tissues that seek to define the mechanisms of normal function and/or disease. Studies of ocular tissues that encompass the disciplines of cell biology, developmental biology, genetics, molecular biology, physiology, biochemistry, biophysics, immunology or microbiology are most welcomed. Manuscripts that are purely clinical or in a surgical area of ophthalmology are not appropriate for submission to Experimental Eye Research and if received will be returned without review.