Anti-Magnaporthe oryzae Activity of Streptomyces bikiniensis HD-087 In Vitro and Bioinformatics Analysis of Polyketide Synthase Gene pksL.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Jiahan Gang, Yuan Ping, Chunmei Du
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Abstract

Streptomyces bikiniensis HD-087 is capable of synthesizing various antimicrobial substances to counter the detrimental effects of hazardous microorganisms. To elucidate whether it produces polyketide antibiotics and the synthesis mechanism of antibiotic substances, the metabolites and related genes of S. bikiniensis HD-087 were analyzed through LC-MS, anti-Magnaporthe oryzae activity detection, and bioinformatics approaches. The result indicated that the strain HD-087 could produce erythromycin, a polyketide antibiotic. The inhibitory zones of the fermentation supernatant of strain HD-087 and methanol solution of erythromycin extract against M. oryzae were 40.84 ± 0.68 mm and 33.18 ± 0.81 mm, respectively. The IC50 value of erythromycin extract for inhibiting spore germination of erythromycin extract was 220.43 μg/mL. There are two polyketide synthesis gene clusters in the genome of strain HD-087, namely t1pks-nrps and t3pks-lantipeptide-t1pks-nrps. The key gene pksL in the t3pks-lantipeptide-t1pks-nrps gene cluster was predicted. The results suggested that it encodes a stable, hydrophilic, and acidic protein, mainly composed of α-helix and random coil. The PksL protein contains dehydrogenase (DH), ketone reductase (KR), acyl carrier protein (ACP), and ketone synthase (KS) domains. Moreover, it can form interaction networks with 11 proteins containing domains, such as polyketide synthase and ACP synthase. The molecular docking between PksL and acetyl-CoA is stable and strong, suggesting that PksL protein could catalyze the synthesis of polyketides with CoA as a substrate. This study provides a theoretical basis for further exploring the polyketides synthesis mechanism and developing antifungal metabolites in S. bikiniensis HD-087.

比基尼链霉菌(Streptomyces bikiniensis)HD-087 的体外抗马齿苋活性和多酮合成酶基因 pksL 的生物信息学分析
比基尼链霉菌(Streptomyces bikiniensis HD-087)能够合成多种抗菌物质,以抵御有害微生物的危害。为了弄清它是否能产生多酮类抗生素以及抗生素物质的合成机制,研究人员通过液相色谱-质谱联用仪、抗Magnaporthe oryzae活性检测和生物信息学方法,分析了比基尼链霉菌HD-087的代谢产物和相关基因。结果表明,HD-087 菌株可生产红霉素(一种多酮类抗生素)。菌株 HD-087 的发酵上清液和红霉素提取物的甲醇溶液对 M. oryzae 的抑制区分别为 40.84 ± 0.68 mm 和 33.18 ± 0.81 mm。红霉素提取物抑制孢子萌发的 IC50 值为 220.43 μg/mL。HD-087菌株基因组中有两个多酮合成基因簇,即t1pks-nrps和t3pks-lantipeptide-t1pks-nrps。预测了 t3pks-lantipeptide-t1pks-nrps 基因簇中的关键基因 pksL。结果表明,它编码一种稳定的亲水性酸性蛋白质,主要由α-螺旋和无规线圈组成。PksL 蛋白含有脱氢酶(DH)、酮还原酶(KR)、酰基载体蛋白(ACP)和酮合成酶(KS)结构域。此外,它还能与多酮合成酶和 ACP 合成酶等 11 种含有结构域的蛋白质形成相互作用网络。PksL与乙酰-CoA之间的分子对接稳定而牢固,表明PksL蛋白可催化以CoA为底物的多酮类化合物的合成。该研究为进一步探索比基尼梭菌HD-087的多酮合成机制和开发抗真菌代谢物提供了理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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