Afaf A Alatawi, Jamaan S Ajarem, Saud A Alarifi, Saleh A Al-Quraishy, Esam M Al-Shaebi, Sarah A Alawwad, Rashed N Herqash, Sally M Khadrawy, Noha A Ahmed, Chuanyi Wang, Saleh N Maodaa
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引用次数: 0
Abstract
Introduction: Tobacco use is a major global health issue linked to psychiatric illnesses and high mortality rates. Nicotine, the primary compound absorbed during smoking, causes harm to various organs, particularly the brain. The current study examined the modulatory effect of Teucrium polium extract (TPE) on nicotine-induced biochemical and histological changes in the brains of mice.
Methods: Twenty-four mice were divided into four groups and were treated for three weeks. Group one was the control; Group two received 100 mg/kg TPE orally; Group three was subcutaneously injected with 2.5 mg/kg nicotine, and Group four received both nicotine and TPE.
Results: The brain tissue of the nicotine-induced group showed histopathological alterations and oxidative stress as indicated by increased lipid peroxidation and nitric oxide levels concomitant with decreased glutathione content and superoxide dismutase activity. DNA fragmentation was also detected by comet assay. Treatment with TPE significantly decreased oxidative stress and DNA fragmentation while increasing antioxidant biomarkers. Histopathological changes were also diminished.
Conclusion: Through the antioxidant activity of TPE, it protected against nicotine-induced neurotoxicity in mice by impacting oxidative stress, DNA fragmentation, and brain histopathological changes.
期刊介绍:
Combinatorial Chemistry & High Throughput Screening (CCHTS) publishes full length original research articles and reviews/mini-reviews dealing with various topics related to chemical biology (High Throughput Screening, Combinatorial Chemistry, Chemoinformatics, Laboratory Automation and Compound management) in advancing drug discovery research. Original research articles and reviews in the following areas are of special interest to the readers of this journal:
Target identification and validation
Assay design, development, miniaturization and comparison
High throughput/high content/in silico screening and associated technologies
Label-free detection technologies and applications
Stem cell technologies
Biomarkers
ADMET/PK/PD methodologies and screening
Probe discovery and development, hit to lead optimization
Combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries)
Chemical library design and chemical diversity
Chemo/bio-informatics, data mining
Compound management
Pharmacognosy
Natural Products Research (Chemistry, Biology and Pharmacology of Natural Products)
Natural Product Analytical Studies
Bipharmaceutical studies of Natural products
Drug repurposing
Data management and statistical analysis
Laboratory automation, robotics, microfluidics, signal detection technologies
Current & Future Institutional Research Profile
Technology transfer, legal and licensing issues
Patents.