Assessment of decellularization strategy and biocompatibility testing of full-thickness abdominal wall to produce a tissue-engineered graft.

IF 1 4区 医学 Q4 ENGINEERING, BIOMEDICAL
George Skepastianos, Panagiotis Mallis, Epameinondas Kostopoulos, Efstathios Michalopoulos, Vasileios Skepastianos, Christos Doudakmanis, Chrysoula Palazi, Gerasimos Tsourouflis
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引用次数: 0

Abstract

Background: Restoration of the abdominal wall defects due to herniation or other complications represents a challenging task of the reconstructive surgery. Synthetic grafts or crosslinked animal-derived grafts, are utilized, followed by significant adverse reactions.

Objective: This study aimed to evaluate primarily the production of a decellularized abdominal wall scaffold and secondly its biocompatibility upon transplantation in an animal model.

Methods: Full-thickness abdominal wall samples were harvested from Wistar Rats and then decellularized utilizing a three-cycle process. To evaluate the decellularization efficacy, histological, biochemical and biomechanical analyses were performed. The biocompatibility assessment involved the implantation of the produced scaffolds to Sprague Dawley rats. The grafts remained for a total period of 4 weeks, followed by immunohistochemistry for the detection of CD11b+, CD4+ and CD8+ cells.

Results: Histological, biochemical and biomechanical results, indicated the production of compatible acellular full-thickness abdominal wall samples. After 4 weeks of implantation, a minor presence of immunity cells was observed.

Conclusion: The data of this study indicated the successful production of a full-thickness abdominal wall scaffold. Biologically derived full-thickness abdominal wall scaffolds may have greater potential in restoration of the abdominal wall defects, bringing them one step closer to their clinical utility.

评估全厚腹壁的脱细胞策略和生物相容性测试,以制作组织工程移植物。
背景:修复因疝气或其他并发症造成的腹壁缺损是整形外科的一项具有挑战性的任务。使用合成移植物或交联动物源移植物会产生严重的不良反应:本研究旨在首先评估脱细胞腹壁支架的生产情况,其次评估其在动物模型中移植时的生物相容性:方法:从 Wistar 大鼠身上采集全厚腹壁样本,然后采用三周期工艺进行脱细胞处理。为评估脱细胞效果,进行了组织学、生物化学和生物力学分析。生物相容性评估包括将制成的支架植入 Sprague Dawley 大鼠体内。移植物共放置了 4 周,然后用免疫组织化学方法检测 CD11b+、CD4+ 和 CD8+ 细胞:结果:组织学、生物化学和生物力学结果表明,制作出了兼容的无细胞全厚腹壁样本。植入 4 周后,观察到少量免疫细胞的存在:结论:这项研究的数据表明,全厚腹壁支架的制作是成功的。生物衍生全厚腹壁支架在修复腹壁缺损方面可能具有更大的潜力,使其离临床应用更近了一步。
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来源期刊
Bio-medical materials and engineering
Bio-medical materials and engineering 工程技术-材料科学:生物材料
CiteScore
1.80
自引率
0.00%
发文量
73
审稿时长
6 months
期刊介绍: The aim of Bio-Medical Materials and Engineering is to promote the welfare of humans and to help them keep healthy. This international journal is an interdisciplinary journal that publishes original research papers, review articles and brief notes on materials and engineering for biological and medical systems. Articles in this peer-reviewed journal cover a wide range of topics, including, but not limited to: Engineering as applied to improving diagnosis, therapy, and prevention of disease and injury, and better substitutes for damaged or disabled human organs; Studies of biomaterial interactions with the human body, bio-compatibility, interfacial and interaction problems; Biomechanical behavior under biological and/or medical conditions; Mechanical and biological properties of membrane biomaterials; Cellular and tissue engineering, physiological, biophysical, biochemical bioengineering aspects; Implant failure fields and degradation of implants. Biomimetics engineering and materials including system analysis as supporter for aged people and as rehabilitation; Bioengineering and materials technology as applied to the decontamination against environmental problems; Biosensors, bioreactors, bioprocess instrumentation and control system; Application to food engineering; Standardization problems on biomaterials and related products; Assessment of reliability and safety of biomedical materials and man-machine systems; and Product liability of biomaterials and related products.
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