First molecular characterisation of Hydatigera taeniaeformis (Cestoda: Taeniidae) from Rattus rattus in urban market centres from Tamale, Ghana.

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Francis Addy, Abdul-Rahman Abubakari, Abdul-Fatawu Mohammed
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Abstract

Background: The common cat tapeworm, Hydatigera taeniaeformis, is widely distributed across many regions of the world but its global genetic diversity, ecology, and other biological features are not fully understood. The predator-prey relationship of felids and rodents sustains the transmission in both sylvatic and synanthropic or urban settings. This study was carried out in three urban market centres in Tamale, Ghana to determine the prevalence of H. taeniaeformis infection in urban rats and characterise the local population genetics using the mitochondrial nad1 gene sequences.

Methods: From January to May 2023, rats were captured and euthanized to identity and isolate the H. taeniaeformis metacestodes. The full mitochondrial nad1 gene was amplified, sequenced and used to characterise the cestode population.

Results: In total 64/80 rats from the three market centres were found naturally infected with metacestodes of H. taeniaeformis. Averagely, infected rats harboured two cysts with one exceptional case of 25 cysts in one liver. Each cyst harboured one metacestode. The DNA sequences revealed four haplotypes in a star-like distribution network across the three market centres. All four haplotypes were found to be unique, clustered with H. taeniaeformis sensu stricto (s.s.) global isolates and had only marginal variation among themselves.

Conclusion: This is the first account of the genetic variability within H. taeniaeformis in Ghana and would contribute to ongoing and future studies on African regional biogeographic distribution of the common cat tapeworm.

从加纳塔马利城市市场中心的老鼠身上首次鉴定 Hydatigera taeniaeformis(绦虫纲:Taeniidae)的分子特征。
背景:常见的猫带绦虫(Hydatigera taeniaeformis)广泛分布于世界许多地区,但人们对其全球遗传多样性、生态学和其他生物学特征并不完全了解。猫科动物和啮齿类动物之间的捕食与被捕食关系维持了这种绦虫在狼群和同类或城市环境中的传播。本研究在加纳塔马利的三个城市市场中心进行,以确定 H. taeniaeformis 在城市老鼠中的感染率,并利用线粒体 nad1 基因序列描述当地种群的遗传学特征:方法:从 2023 年 1 月至 5 月,捕获并安乐死大鼠,以鉴定和分离 H. taeniaeformis metacestodes。对线粒体 nad1 全基因进行扩增、测序,并用于描述绦虫种群的特征:结果:三个市场中心共有 64/80 只老鼠自然感染了 H. taeniaeformis 元绦虫。平均而言,受感染的老鼠身上有两个囊肿,其中一个特殊病例的一个肝脏内有 25 个囊肿。每个囊肿都有一个元孢子虫。DNA 序列显示,在三个市场中心的星状分布网络中有四个单倍型。所有四个单倍型都是独特的,与严格意义上的 H. taeniaeformis(s.s.)全球分离株聚集在一起,它们之间的差异很小:这是对加纳 H. taeniaeformis 遗传变异的首次描述,有助于当前和未来对非洲地区常见猫带绦虫生物地理分布的研究。
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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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