Engineered probiotic E.coli Nissle 1917 for release PTEN to improve the tumor microenvironment and suppress tumor growth.

IF 2 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-09-27 DOI:10.1007/s10529-024-03536-6
Zirui Dai, Wenjuan Zhao, Li Cao, Zirong Zhu, Ziyuan Xia, Liqiu Xia
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引用次数: 0

Abstract

The cancer is one of the diseases of serious threat to people's health and life nowadays. But heterogeneity, drug resistance and treatment side effects of cancer, traditional treatments still have limitations. Tumor-targeting probiotics with a well-established Biosafety and efficient targeting as a delivery vectors to deliver anticancer genes or antitumor drugs to tumor microenvironment has attracted much attention in cancer therapies. In this study, E.coil Nissle 1917 (EcN) was utilized to deliver eukaryotic anti-tumor protein PTEN to tumor microenvironment and suppress tumor growth. Therefore, the EcN (PTEN) was developed. Our results demonstrated that EcN (PTEN) could colonize the tumor site accurately and inhibit the growth of colorectal cancer cells in tumor-bearing mice. It is worth noting that the tumor microenvironment of the treated mice showed significant recruitment of and M1 macrophages, neutrophils and T lymphocytes. No toxicity was observed in the normal tissues during the experiments. This research show the probiotic EcN(PTEN) holds the promise of becoming a powerful weapon against cancer and expected to provide more effective treatments for cancer patients.

设计益生菌大肠杆菌 Nissle 1917 以释放 PTEN,从而改善肿瘤微环境并抑制肿瘤生长。
癌症是当今严重威胁人类健康和生命的疾病之一。但由于癌症的异质性、耐药性和治疗副作用,传统的治疗方法仍然存在局限性。肿瘤靶向益生菌具有良好的生物安全性和高效的靶向性,可作为递送载体将抗癌基因或抗肿瘤药物递送到肿瘤微环境中,在癌症治疗中备受关注。本研究利用 E.coil Nissle 1917(EcN)将真核抗肿瘤蛋白 PTEN 运送到肿瘤微环境中并抑制肿瘤生长。因此,EcN(PTEN)应运而生。我们的研究结果表明,EcN(PTEN)能准确定植于肿瘤部位,并抑制肿瘤小鼠体内结直肠癌细胞的生长。值得注意的是,接受治疗的小鼠的肿瘤微环境中出现了大量的 M1 巨噬细胞、中性粒细胞和 T 淋巴细胞。在实验过程中,正常组织未观察到任何毒性。这项研究表明,益生菌EcN(PTEN)有望成为抗癌的有力武器,并有望为癌症患者提供更有效的治疗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biotechnology Letters
Biotechnology Letters 工程技术-生物工程与应用微生物
CiteScore
5.90
自引率
3.70%
发文量
108
审稿时长
1.2 months
期刊介绍: Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.
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