Determination of drugs of abuse and metabolites in plasma microsamples by LC-MS/MS after microQuEChERS extraction.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-09-27 DOI:10.1080/17576180.2024.2404383

Mariana Smidt, Marcos Frank Bastiani, Roberta Zilles Hahn, Lilian de Lima Feltraco Lizot, Amanda Pacheco Bondan, Giovana Piva Peteffi, Náthaly Cbf Dos Santos, José Luiz da Costa, Rafael Lanaro, Rafael Linden

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引用次数: 0

Abstract

Aim: Identifying drugs of abuse and their metabolites in plasma is vital in both forensic and clinical toxicology. While the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method offers an efficient approach to sample preparation, its application is complex due to the wide-ranging properties of target analytes and the challenges posed by biological matrix interferences. This study aims to develop a microQuEChERS approach for the quantification of 14 drugs of abuse and metabolites utilizing minimal sample and solvent volumes.Methods: The microQuEChERS method involved using 10 μl plasma samples, 25 mg of a salt mixture and 150 μl of acetonitrile. Extracts were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS), with a 7.5 min run. The assay was validated according to bioanalytical guidelines.Results: The accuracy was 96.8-112.4%. The within-assay precision was within 2.0-8.9% and the between-assay precision was within 3.2-8.2%. Matrix effects were found to range from -5.7 to 13.5%. The extraction yield was higher than 74.7%.Conclusion: This study described a microQuEChERS sample preparation approach for determining drugs of abuse and metabolites using plasma microsamples and LC-MS/MS. The approach is efficient, environmentally friendly and suitable for scenarios with limited amounts of biological samples.

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微量 QuEChERS 萃取后通过 LC-MS/MS 测定血浆微量样本中的滥用药物和代谢物。

目的：鉴别血浆中的滥用药物及其代谢物对法医和临床毒理学都至关重要。QuEChERS（快速、简便、廉价、有效、坚固和安全）方法提供了一种高效的样品制备方法，但由于目标分析物的各种特性以及生物基质干扰带来的挑战，该方法的应用非常复杂。本研究旨在开发一种微型 QuEChERS 方法，利用最小的样品和溶剂用量对 14 种滥用药物和代谢物进行定量分析：微量 QuEChERS 方法包括使用 10 μl 血浆样品、25 毫克盐混合物和 150 μl 乙腈。提取物采用液相色谱-串联质谱法（LC-MS/MS）进行分析，运行时间为 7.5 分钟。根据生物分析指南对该检测方法进行了验证：结果：准确度为 96.8%-112.4%。测定内精密度为 2.0-8.9%，测定间精密度为 3.2-8.2%。基质效应为-5.7%至13.5%。萃取率高于 74.7%：本研究介绍了一种利用血浆微量样品和 LC-MS/MS 测定药物滥用和代谢物的 microQuEChERS 样品制备方法。该方法高效、环保，适用于生物样本量有限的情况。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.