Oocytes Vitrification Using Automated Equipment Based on Microfluidic Chip.

IF 3 2区 医学 Q3 ENGINEERING, BIOMEDICAL
Jing Shen, Zixuan Yu, Weijie Li, Xinli Zhou
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引用次数: 0

Abstract

Oocyte vitrification has a wide range of applications in assisted reproduction and fertility preservation. It requires precise cryoprotectant agents (CPAs) loading and removal sequences to alleviate osmotic shock, which requires manual manipulation by an embryologist. In this study, a microfluidic system was developed to facilitate the precise adjustment of the CPA concentration around the oocyte by linear loading and removal of CPA. In addition, the microfluidic-based automated vitrification (MAV) device combines CPA loading/removal process, with vitrification process, thereby achieving automated oocyte vitrification. Oocytes were vitrified by Cryotop/QC manual method and MAV method. The results showed that the survival, cleavage, and blastocyst rates of oocytes were 80.44, 54.17, and 32.95% for the MAV method, which were significantly higher than Cryotop manual method (73.35, 43.73, and 23.67%) (p < 0.05). In MAV, solution injection rate during CPA loading/removal process was designed as a 1-segment, 2-segment, and 4-segment function. Accordingly, three concave loading and convex removal protocols were adopted to vitrify oocytes. Oocytes vitrified using the 4-segment function group exhibited increased survival (86.18%), cleavage (63.29%), and blastocyst (45.58%) rates compared to those vitrified using the 1-segment and 2-segment groups. The oocytes vitrification with the highest concentration of CPA, denoted as VS1-TS1, exhibited the highest survival rate after rewarming (86.18%). In contrast, the VS3-TS3 group, characterized by a CPA concentration half that of VS1-TS1, exhibited lower survival (74.14%) and cleavage (59.31%) rates, but displayed the higher blastocyst rate (50.79%) following oocyte activation. Our study demonstrates potential of the MAV device for oocyte or embryo vitrification.

使用基于微流控芯片的自动化设备进行卵母细胞玻璃化。
卵母细胞玻璃化技术在辅助生殖和生育力保存方面有着广泛的应用。它需要精确的冷冻保护剂(CPA)加载和移除顺序来减轻渗透休克,这需要胚胎学家手动操作。本研究开发了一种微流体系统,可通过线性加载和移除 CPA 来精确调节卵母细胞周围的 CPA 浓度。此外,基于微流体的自动玻璃化(MAV)装置将 CPA 的装载/移除过程与玻璃化过程相结合,从而实现了卵母细胞的自动玻璃化。采用 Cryotop/QC 手动方法和 MAV 方法对卵母细胞进行玻璃化。结果显示,MAV 方法的卵母细胞存活率、裂解率和囊胚率分别为 80.44%、54.17% 和 32.95%,明显高于 Cryotop 手动方法(73.35%、43.73% 和 23.67%)(p
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来源期刊
Annals of Biomedical Engineering
Annals of Biomedical Engineering 工程技术-工程:生物医学
CiteScore
7.50
自引率
15.80%
发文量
212
审稿时长
3 months
期刊介绍: Annals of Biomedical Engineering is an official journal of the Biomedical Engineering Society, publishing original articles in the major fields of bioengineering and biomedical engineering. The Annals is an interdisciplinary and international journal with the aim to highlight integrated approaches to the solutions of biological and biomedical problems.
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