The effect of citric acid on mineralisation and vascular endothelial growth factor secretion from apical papilla stem cells.

IF 1.4 4区医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Acta Odontologica Scandinavica Pub Date : 2024-10-01 DOI:10.2340/aos.v83.42026

Krasimir Hristov, Nikolay Ishkitiev, Marina Miteva, Violeta Dimitrova, Ralitsa Gigova, Nataliya Gateva, Liliya Angelova

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Abstract

Objective: To investigate the influence of citric acid on the osteogenic and angiogenic potential of stem cells from apical papillae (SCAPs).

Materials and methods: Stem cells from apical papillae were isolated from freshly extracted third permanent molars. These cells were treated with 20 and 100 μM citric acid. Alizarin red staining was used to evaluate mineral deposition. The secreted levels of vascular endothelial growth factor (VEGF) were assessed by ELISA on days 18, 24 and 28. Immunofluorescence analysis was performed to assess the expression of surface markers after exposure to 20 and 100 μM citric acid.

Results: Different mineralisation patterns were observed. Supplemented with citric acid, media showed more diffuse and less dense crystals. On day 18, most VEGF was secreted from the cells with no added citric acid. On day 24, there was a significant increase (p < 0.05) in the levels of VEGF secreted from cells treated with 20 μM citric acid. On day 28, cells from the control group did not secrete VEGF. There was a reduction in the levels of VEGF secreted by cells treated with 20 μM citric acid and a significant increase in the cells exposed to 100 μM citric acid (p < 0.05).

Conclusion: Citric acid can promote the differentiation of SCAPs and angiogenesis.

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柠檬酸对顶端乳头干细胞矿化和血管内皮生长因子分泌的影响

目的：研究柠檬酸对根尖乳头干细胞成骨和血管生成潜能的影响：研究柠檬酸对根尖乳头干细胞（SCAPs）成骨和血管生成潜能的影响：从新鲜拔出的第三恒磨牙中分离出牙根尖乳头干细胞。用 20 和 100 μM 柠檬酸处理这些细胞。茜素红染色用于评估矿物质沉积。在第 18、24 和 28 天，用 ELISA 方法评估血管内皮生长因子（VEGF）的分泌水平。免疫荧光分析用于评估暴露于 20 μM 和 100 μM 柠檬酸后表面标记物的表达：结果：观察到不同的矿化模式。补充柠檬酸后，培养基显示出更多弥散的晶体，密度较低。第 18 天，大部分 VEGF 从未加柠檬酸的细胞中分泌出来。第 24 天，经 20 μM 柠檬酸处理的细胞分泌的 VEGF 水平显著增加（p < 0.05）。第 28 天，对照组细胞不分泌 VEGF。经 20 μM 柠檬酸处理的细胞分泌的血管内皮生长因子水平有所下降，而暴露于 100 μM 柠檬酸的细胞分泌的血管内皮生长因子水平显著上升（p < 0.05）：柠檬酸可促进SCAPs分化和血管生成。

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