Upcycling of tea processing waste into kombucha-derived bioactive cellulosic composite for prospective wound dressing action.

IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
3 Biotech Pub Date : 2024-10-01 Epub Date: 2024-09-27 DOI:10.1007/s13205-024-04095-3
Baishali Dey, Sivaraman Jayaraman, Paramasivan Balasubramanian
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引用次数: 0

Abstract

The aim of the study was to utilize kombucha-derived bacterial cellulosic sheet [KBC], formed as a by-product of fermented, sugared black tea (in the presence of a symbiotic culture of bacteria and yeast), for potential wound dressing applications. KBC was functionalized using aqueous and ethanolic extracts of different phytochemical agents using two ex-situ methods- casting and impregnation. It was observed that casted KBC functionalized with ethanolic extract of Turmeric (1.2% w/w) yielded a maximum zone of inhibition (24.37 ± 0.42 mm) against Pseudomonas aeruginosa. The hemocompatibility test confirmed the composite's biocompatible nature as the percentage hemocompatibility was found to be less than 5%. The MTT assay established its viability and anti-cancerous properties with Turmeric extract loaded KBC showing higher efficiency compared to Tulsi extract. FTIR analysis and SEM imaging confirmed the functionalization of cellulose sheets and the change in morphology. The contact angle analysis showed improved hydrophilic properties of the sheets for absorbing wound exudates, and the water absorption study revealed maximum absorptivity of up to 321.20 ± 6.23%. Thus, it can be concluded from the study that tea processing waste can be reused to produce a value-added product that can act as an efficient, cost-effective biomaterial for wound dressing applications.

将茶叶加工废料升级再造为从昆布茶中提取的生物活性纤维素复合材料,用于伤口敷料。
该研究旨在利用昆布茶衍生的细菌纤维素薄片 [KBC](在细菌和酵母共生培养的情况下)作为发酵糖渍红茶的副产品,用于潜在的伤口敷料应用。采用两种原位方法--浇铸法和浸渍法,利用不同植物化学制剂的水提取物和乙醇提取物对 KBC 进行了功能化处理。结果表明,用姜黄乙醇提取物(1.2% w/w)制成的功能化 KBC 对铜绿假单胞菌的抑制区最大(24.37 ± 0.42 mm)。血液相容性测试证实了该复合材料的生物相容性,其血液相容性低于 5%。MTT 试验证实了 KBC 的活力和抗癌特性,与涂尔西提取物相比,负载姜黄提取物的 KBC 表现出更高的效率。傅立叶变换红外光谱分析和扫描电镜成像证实了纤维素片的功能化和形态变化。接触角分析表明,纤维素片在吸收伤口渗出物方面具有更好的亲水性,吸水率研究表明,最大吸水率可达 321.20 ± 6.23%。因此,这项研究得出结论,茶叶加工废料可以再利用,生产出高附加值的产品,作为一种高效、经济的生物材料用于伤口敷料。
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来源期刊
3 Biotech
3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍: 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.
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