Novel electrochemiluminescence platform utilizing AuNPs@Uio-66-NH₂ bridged luminescent substrates and aptamers for the detection of pesticide residues in Chinese herbal medicines.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-01-01 Epub Date: 2024-09-20 DOI:10.1016/j.talanta.2024.126924

Chengqiang Li, Haifang Wang, Jiashuai Sun, Peisen Li, Jiwei Dong, Jingcheng Huang, Haowei Dong, Lingjun Geng, Zhiping Yu, Pengwei Zhang, Wei Chen, Yemin Guo, Xia Sun

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引用次数: 0

Abstract

A large number of Chinese herbal medicines (CHMs) are included in daily recipes, but their pesticide residues have aroused more and more concerns. In this paper, an electrochemiluminescence aptasensor was constructed for the trace detection of acetamiprid (ACE) in Angelica sinensis and Lycium barbarum. Possessing a large specific surface area, UiO-66 was modified with amino groups to improve biocompatibility, and the addition of AuNPs allowed UiO-66-NH₂ to catalyze the formation of excited states of luminescent molecules (TPrA^⁎; Ru(bpy)₃²⁺^⁎), and AuNPs@UiO-66-NH₂ was used to bridge the aptamer (Au-S) and luminescent substrate (peptide bond). The conventional luminescent reagent Ru(bpy)₃²⁺ was doped with multi-walled carbon nanotubes (MWCNTs) to obtain a more powerful and stable light signal. After optimizing the experimental parameters, the aptasensor could give results in 10 min with a detection range from 1×10^-2-1×10⁴ nM and a lower limit of detection (LOD) of 0.8 pM. The LOD of the study was at least one order of magnitude lower than that of the fluorescence detection method. Furthermore, the accuracy of the aptasensor was validated for spiked recovery experiments.

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利用 AuNPs@Uio-66-NH2 桥接发光底物和适配体的新型电化学发光平台检测中药材中的农药残留。

大量中药材被列入日常食谱，但其农药残留问题日益引起人们的关注。本文构建了一种电化学发光适配传感器，用于痕量检测当归和枸杞中的啶虫脒（ACE）。UiO-66 具有较大的比表面积，用氨基修饰可提高其生物相容性，加入 AuNPs 可使 UiO-66-NH2 催化发光分子（TPrA⁎；Ru(py)32+⁎）激发态的形成，AuNPs@UiO-66-NH2 则用于连接适配体（Au-S）和发光底物（肽键）。在传统的发光试剂 Ru(bpy)32+ 中掺杂了多壁碳纳米管（MWCNTs），以获得更强更稳定的光信号。优化实验参数后，该传感器可在 10 分钟内得到结果，检测范围为 1×10-2-1×104 nM，检测下限（LOD）为 0.8 pM。该研究的检测下限比荧光检测方法至少低一个数量级。此外，在加标回收实验中也验证了该传感器的准确性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.