Enhanced performance of impedimetric immunosensors to detect SARS-CoV-2 with bare gold nanoparticles and graphene acetic acid.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-01-01 Epub Date: 2024-09-25 DOI:10.1016/j.talanta.2024.126903

Rafael C Hensel, Biagio Di Vizio, Elsa M Materòn, Flávio M Shimizu, Monara Kaelle S C Angelim, Gabriela F de Souza, José L P Módena, Pedro M M Moraes-Vieira, Ricardo B de Azevedo, Lucio Litti, Stefano Agnoli, Stefano Casalini, Osvaldo N Oliveira

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引用次数: 0

Abstract

Immunosensors based on electrical impedance spectroscopy allow for label-free, real-time detection of biologically relevant molecules and pathogens, without requiring electro-active materials. Here, we investigate the influence of bare gold nanoparticles (AuNPs), synthesized via laser ablation in solution, on the performance of an impedimetric immunosensor for detecting severe acute respiratory syndrome coronavirus (SARS-CoV-2). Graphene acetic acid (GAA) was used in the active layer for immobilizing anti-SARS-CoV-2 antibodies, owing to its high density of carboxylic groups. Immunosensors incorporating AuNPs exhibited superior performance compared to those relying solely on GAA, achieving a limit of detection (LoD) of 3 x 10^-20 g/mL to detect the Spike Receptor Binding Domain (RBD) protein of SARS-CoV-2 and of 2 PFU/mL for inactivated virus. Moreover, these immunosensors presented high selectivity against the H1N1 influenza virus. We anticipate that this platform will be versatile and applicable in the early diagnosis of various diseases and viral infections, thereby facilitating Point-of-Care testing.

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利用裸金纳米粒子和醋酸石墨烯提高阻抗免疫传感器检测 SARS-CoV-2 的性能。

基于电阻抗光谱的免疫传感器无需电活性材料，即可对生物相关分子和病原体进行无标记实时检测。在此，我们研究了在溶液中通过激光烧蚀合成的裸金纳米粒子（AuNPs）对阻抗免疫传感器性能的影响，该传感器用于检测严重急性呼吸系统综合征冠状病毒（SARS-CoV-2）。石墨烯乙酸（GAA）具有高密度的羧基，因此被用于固定抗 SARS-CoV-2 抗体的活性层。与仅依赖 GAA 的免疫传感器相比，含有 AuNPs 的免疫传感器表现出更优越的性能，检测 SARS-CoV-2 的尖峰受体结合域（RBD）蛋白的检测限（LoD）为 3 x 10-20 g/mL，检测灭活病毒的检测限（LoD）为 2 PFU/mL。此外，这些免疫传感器对 H1N1 流感病毒具有高选择性。我们预计该平台将具有多功能性，可用于各种疾病和病毒感染的早期诊断，从而促进护理点检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.