Electrochemiluminescence immunoassay system based on PCN-224-Mn and gold-platinum bimetallic nanoflowers for sensitive detection of ochratoxin A.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-01-01 Epub Date: 2024-09-24 DOI:10.1016/j.talanta.2024.126937

Yue Kong, Xinyue Qian, Xiao Mei, Jun Ma, Kang Wu, Anping Deng, Jianguo Li

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引用次数: 0

Abstract

In this work, a novel Electrochemiluminescence Immunosensor was constructed using PCN-224-Mn and gold-platinum nanoflowers (AuPt NFs) for the ultrasensitive detection of ochratoxin A (OTA). PCN-224 modified with Mn (II) was synthesized as a probe material. The interaction efficiency of PCN-224 with S₂O₈^2- was also greatly improved. AuPt NFs were used as the substrate material for the electrodes. It has favorable biocompatibility, large specific surface area and can bind more antigen. Also greatly increased the electroactive surface area and conductivity of the electrode. OTA was detected using a competitive immunoassay strategy, in which OTA in the sample competes with the encapsulated antigen for a finite number of antibodies. ECLIA for the detection of OTA was designed to be highly sensitive, with a linear range from 0.0002 ng mL^-1 to 1000 ng mL^-1 and a LOD as low as 0.067 pg mL^-1. In addition, it was evident from the electrochemical analyses that PCN-224-Mn had a stronger and more stable ECL signal compared to the plain PCN-224. The successful preparation of specific, sensitive and reproducible ECL immunosensors confirms the great promise for the detection of OTA or other small molecule mycotoxins.

查看原文本刊更多论文

基于 PCN-224-Mn 和金铂双金属纳米流的电化学发光免疫分析系统，用于灵敏检测赭曲霉毒素 A。

本研究利用 PCN-224-Mn 和金铂纳米花（AuPt NFs）构建了一种新型电化学发光免疫传感器，用于超灵敏检测赭曲霉毒素 A（OTA）。用 Mn (II) 修饰的 PCN-224 被合成为探针材料。PCN-224 与 S2O82- 的相互作用效率也大大提高。AuPt NFs 被用作电极的基底材料。它具有良好的生物相容性，比表面积大，能结合更多的抗原。同时还大大增加了电极的电活性表面积和电导率。采用竞争性免疫测定策略检测 OTA，即样品中的 OTA 与封装的抗原竞争有限数量的抗体。用于检测 OTA 的 ECLIA 设计为高灵敏度，线性范围为 0.0002 ng mL-1 至 1000 ng mL-1，LOD 低至 0.067 pg mL-1。此外，电化学分析表明，与普通 PCN-224 相比，PCN-224-Mn 的 ECL 信号更强、更稳定。特异性、灵敏性和可重复性 ECL 免疫传感器的成功制备证实了其在检测 OTA 或其他小分子霉菌毒素方面的巨大前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.