Transcriptome Analysis Revealed the Anabolic Regulation of Chlorophyll and Carotenoids in Curcuma alismatifolia Bracts.

IF 2.1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Genetics Pub Date : 2024-09-26 DOI:10.1007/s10528-024-10923-1

Suhua Jiang, Shaoqiang Tu, Lingjun Ke, Luanmei Lu, Huiwen Yu

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引用次数: 0

Abstract

Curcuma alismatifolia is an attractive ornamental plant in the ginger family. Its bracts come in a variety of colors and are commonly used as cut flowers, potted plants, and landscaping. To investigate the regulation of bract pigmentation in C. alismatifolia, we examined the pigment levels of chlorophyll and carotenoids in the pure color part (PC) and variegated part (VA) of three C. alismatifolia varieties, i.e., "Siam TM Sitrone," "Chiang Mai Pink," and "Snow White." To mine the color mechanisms of the pure color and variegated parts of the bract, we conducted RNA-seq analysis on C. alismatifolia. We identified a total of 89,975 unigenes, and there were 3584 differentially expressed genes identified post-screening. Furthermore, 1858 DEGs were annotated in the GO database and 681 in the KEGG database. We pinpointed key genes responsible for the diverse bract colors in C. alismatifolia, including ZEP for carotenoid synthesis and GAGA2 in the chlorophyll synthesis pathway. This study provides valuable insights into understanding the pigmentation mechanism of bracts in C. alismatifolia and the breeding process.

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转录组分析揭示了莪术苞片中叶绿素和类胡萝卜素的合成代谢调控。

Curcuma alismatifolia 是姜科植物中一种极具吸引力的观赏植物。它的苞片有多种颜色，通常用作切花、盆栽和园林景观。为了研究枸橘苞片色素沉着的调节机制，我们研究了三个枸橘品种（即 "暹罗 TM Sitrone"、"清迈粉红 "和 "雪白"）的纯色部分（PC）和变色部分（VA）的叶绿素和类胡萝卜素的色素含量。为了挖掘苞片纯色部分和变色部分的颜色机制，我们对 C. alismatifolia 进行了 RNA-seq 分析。我们共鉴定了 89,975 个单基因，筛选后发现了 3584 个差异表达基因。此外，在 GO 数据库中注释了 1858 个 DEGs，在 KEGG 数据库中注释了 681 个 DEGs。我们确定了造成 C. alismatifolia 不同苞片颜色的关键基因，包括合成类胡萝卜素的 ZEP 和叶绿素合成途径中的 GAGA2。这项研究为了解 C. alismatifolia 苞片的色素形成机制和育种过程提供了宝贵的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemical Genetics 生物-生化与分子生物学

CiteScore

3.90

自引率

0.00%

发文量

133

审稿时长

4.8 months

期刊介绍： Biochemical Genetics welcomes original manuscripts that address and test clear scientific hypotheses, are directed to a broad scientific audience, and clearly contribute to the advancement of the field through the use of sound sampling or experimental design, reliable analytical methodologies and robust statistical analyses. Although studies focusing on particular regions and target organisms are welcome, it is not the journal’s goal to publish essentially descriptive studies that provide results with narrow applicability, or are based on very small samples or pseudoreplication. Rather, Biochemical Genetics welcomes review articles that go beyond summarizing previous publications and create added value through the systematic analysis and critique of the current state of knowledge or by conducting meta-analyses. Methodological articles are also within the scope of Biological Genetics, particularly when new laboratory techniques or computational approaches are fully described and thoroughly compared with the existing benchmark methods. Biochemical Genetics welcomes articles on the following topics: Genomics; Proteomics; Population genetics; Phylogenetics; Metagenomics; Microbial genetics; Genetics and evolution of wild and cultivated plants; Animal genetics and evolution; Human genetics and evolution; Genetic disorders; Genetic markers of diseases; Gene technology and therapy; Experimental and analytical methods; Statistical and computational methods.