Integrated genomic, transcriptomic, and metabolomic analyses of Ilex hylonoma provide insights into the triterpenoid saponin biosynthesis.

IF 6.2 1区 生物学 Q1 PLANT SCIENCES
The Plant Journal Pub Date : 2024-11-01 Epub Date: 2024-09-27 DOI:10.1111/tpj.17046
Landi Feng, Yingjun Yao, Minghui Kang, Wengjie Yang, Yu Han, Wei Liu, Xiaonan Li, Na Li, Yongqi Hu, Jianquan Liu, Quanjun Hu
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引用次数: 0

Abstract

Ilex is known for its rich content of secondary metabolites, particularly triterpenoid saponins. These compounds hold significant value in natural remedies and herbal medicine. However, the molecular mechanisms responsible for triterpenoid biosynthesis in plants of this genus remain largely unexplored. In this study, we successfully generated the first chromosome-scale genome of Ilex hylonoma. The assembly, comprising 20 anchored chromosomes, has an N50 contig size of 2.13 Mb and a scaffold size of 33.68 Mb. Comparative genome analyses with two other congeners with available chromosome-level genomes suggested that an end-to-end chromosome fusion event likely contributed to the reduction in chromosome number from n = 20 to n = 19 within this genus. By integrating transcriptomic and metabolomic data, we identified the gene expression patterns and metabolite profiles of I. hylonoma across three commonly utilized medicinal tissues. We subsequently pinpointed candidate genes involved in the regulation of triterpenoid saponin biosynthesis, including CYP450 genes, UGT genes, and associated transcription factors. Furthermore, yeast heterologous expression analysis revealed that ihyl08363 catalyzed the conversion of β-amyrin into oleanolic acid, while ihyl04303 catalyzed the C-2α hydroxylation of oleanolic acid to produce maslinic acid. This integrated analysis provides valuable insights into the biosynthesis of important triterpenoid saponins in medicinal Ilex plants.

对 Ilex hylonoma 的基因组、转录组和代谢组进行综合分析,有助于深入了解三萜类皂苷的生物合成。
Ilex 以其丰富的次生代谢物含量而闻名,尤其是三萜类皂甙。这些化合物在天然疗法和草药方面具有重要价值。然而,该属植物中三萜类生物合成的分子机制在很大程度上仍未得到探索。在这项研究中,我们成功生成了 Ilex hylonoma 的首个染色体级基因组。该基因组由 20 条固定染色体组成,N50 等位基因大小为 2.13 Mb,支架大小为 33.68 Mb。与其他两个具有染色体级基因组的同属植物进行的基因组比较分析表明,端对端染色体融合事件很可能是导致该属植物染色体数从 n = 20 减少到 n = 19 的原因。通过整合转录组和代谢组数据,我们确定了 I. hylonoma 在三种常用药用组织中的基因表达模式和代谢物特征。随后,我们确定了参与调控三萜类皂苷生物合成的候选基因,包括 CYP450 基因、UGT 基因和相关转录因子。此外,酵母异源表达分析表明,ihyl08363 催化 β-amyrin 转化为齐墩果酸,而 ihyl04303 催化齐墩果酸的 C-2α 羟基化生成马斯林酸。这项综合分析为了解药用 Ilex 植物中重要的三萜类皂苷的生物合成提供了宝贵的信息。
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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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