Light signaling-dependent regulation of plastid RNA processing in Arabidopsis.

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Lili Hu, Qian Wu, Chunyu Wu, Chunmei Zhang, Ziying Wu, Meihui Shi, Man Zhang, Sujuan Duan, Hong-Bin Wang, Hong-Lei Jin
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引用次数: 0

Abstract

Light is a vital environmental signal that regulates the expression of plastid genes. Plastids are crucial organelles that respond to light, but the effects of light on plastid RNA processing following transcription remain unclear. In this study, we systematically examined the influence of light exposure on plastid RNA processing, focusing on RNA splicing and RNA editing. We demonstrated that light promotes the splicing of transcripts from the plastid genes rps12, ndhA, atpF, petB, and rpl2. Additionally, light increased the editing rate of the accD transcript at nucleotide 794 (accD-794) and the ndhF transcript at nucleotide 290 (ndhF-290), while decreasing the editing rate of the clpP transcript at nucleotide 559 (clpP-559). We have identified key regulators of signaling pathways, such as CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1), ELONGATED HYPOCOTYL 5 (HY5), and PHYTOCHROME-INTERACTING FACTORs (PIFs), as important players in the regulation of plastid RNA splicing and editing. Notably, COP1 was required for GENOMES UNCOUPLED1 (GUN1)-dependent repression of clpP-559 editing in the light. We showed that HY5 and PIF1 bind to the promoters of nuclear genes encoding plastid-localized RNA processing factors in a light-dependent manner. This study provides insight into the mechanisms underlying light-mediated post-transcriptional regulation of plastid gene expression.

拟南芥质体 RNA 处理的光信号依赖性调控。
光是调节质体基因表达的重要环境信号。质体是对光做出反应的关键细胞器,但光对转录后质体 RNA 处理的影响仍不清楚。在这项研究中,我们系统地研究了光照对质体 RNA 处理的影响,重点是 RNA 剪接和 RNA 编辑。我们证实,光能促进质体基因 rps12、ndhA、atpF、petB 和 rpl2 的转录本剪接。此外,光还提高了核苷酸 794(accD-794)处的 accD 转录本和核苷酸 290(ndhF-290)处的 ndhF 转录本的剪接率,同时降低了核苷酸 559(clpP-559)处的 clpP 转录本的剪接率。我们发现信号通路的关键调控因子,如CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1)、ELONGATED HYPOCOTYL 5 (HY5)和PHYTOCHROME-INTERACTING FACTORs (PIFs),是调节质体RNA剪接和编辑的重要角色。值得注意的是,COP1 是 GENOMES UNCOUPLED1 (GUN1) 依赖性抑制 clpP-559 在光下编辑所必需的。我们发现,HY5 和 PIF1 以光照依赖的方式与编码质体定位 RNA 处理因子的核基因启动子结合。这项研究深入揭示了光介导的转录后质体基因表达调控机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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