Variations in the germinal center response revealed by genetically diverse mouse strains

IF 3.2 4区 医学 Q3 CELL BIOLOGY
Anne-Marie Aubin, Daria Vdovenko, Roxanne Collin, Lois Balmer, Lise Coderre, Grant Morahan, Félix Lombard-Vadnais, Sylvie Lesage
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引用次数: 0

Abstract

The humoral response is complex and involves multiple cellular populations and signaling pathways. Bacterial and viral infections, as well as immunization regimens, can trigger this type of response, promoting the formation of microanatomical cellular structures called germinal centers (GCs). GCs formed in secondary lymphoid organs support the differentiation of high-affinity plasma cells and memory B cells. There is growing evidence that the quality of the humoral response is influenced by genetic variants. Using 12 genetically divergent mouse strains, we assessed the impact of genetics on GC cellular traits. At steady state, in the spleen, lymph nodes and Peyer's patches, we quantified GC B cells, plasma cells and follicular helper T cells. These traits were also quantified in the spleen of mice following immunization with a foreign antigen, namely, sheep red blood cells, in addition to the number and size of GCs. We observed both strain- and organ-specific variations in cell type abundance, as well as for GC number and size. Moreover, we find that some of these traits are highly heritable. Importantly, the results of this study inform on the impact of genetic diversity in shaping the GC response and identify the traits that are the most impacted by genetic background.

Abstract Image

基因不同的小鼠品系揭示了生殖中心反应的变异。
体液反应非常复杂,涉及多个细胞群和信号通路。细菌和病毒感染以及免疫接种方案都会触发这类反应,促进形成称为生殖中心(GCs)的微解剖细胞结构。在次级淋巴器官中形成的生殖中心支持高亲和性浆细胞和记忆 B 细胞的分化。越来越多的证据表明,体液反应的质量受基因变异的影响。我们利用 12 个基因不同的小鼠品系,评估了遗传对 GC 细胞特征的影响。在稳定状态下,我们对脾脏、淋巴结和派尔斑块中的GC B细胞、浆细胞和滤泡辅助T细胞进行了量化。在小鼠脾脏免疫外来抗原(即绵羊红细胞)后,除了GC的数量和大小外,我们还对这些特征进行了量化。我们观察到了细胞类型丰度以及 GC 数量和大小的菌株和器官特异性变化。此外,我们还发现其中一些性状具有高度遗传性。重要的是,这项研究的结果说明了遗传多样性对形成 GC 反应的影响,并确定了受遗传背景影响最大的性状。
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来源期刊
Immunology & Cell Biology
Immunology & Cell Biology 医学-免疫学
CiteScore
7.50
自引率
2.50%
发文量
98
审稿时长
4-8 weeks
期刊介绍: The Australasian Society for Immunology Incorporated (ASI) was created by the amalgamation in 1991 of the Australian Society for Immunology, formed in 1970, and the New Zealand Society for Immunology, formed in 1975. The aim of the Society is to encourage and support the discipline of immunology in the Australasian region. It is a broadly based Society, embracing clinical and experimental, cellular and molecular immunology in humans and animals. The Society provides a network for the exchange of information and for collaboration within Australia, New Zealand and overseas. ASI members have been prominent in advancing biological and medical research worldwide. We seek to encourage the study of immunology in Australia and New Zealand and are active in introducing young scientists to the discipline.
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