Mixed species biofilms act as planktonic cell factories despite isothiazolinone exposure under continuous-flow conditions

IF 3.6 4区生物学 Q2 ENVIRONMENTAL SCIENCES

Environmental Microbiology Reports Pub Date : 2024-10-01 DOI:10.1111/1758-2229.70010

Kyle B. Klopper, Elanna Bester, Martha van Schalkwyk, Gideon M. Wolfaardt

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Abstract

The primary approach to managing biofouling in industrial water systems involves the large-scale use of biocides. It is well-established that biofilms are ‘cell factories’ that release planktonic cells even when challenged with antimicrobials. The effect of isothiazolinone on the metabolic activity and biomass of mixed Pseudomonas biofilms was monitored in real-time using the CEMS-BioSpec system. The exposure of biofilms to the minimum inhibitory concentration (1.25 mg L⁻¹) of biocide did not impact planktonic cell production (log 7.5 CFU mL⁻¹), while whole-biofilm metabolic activity and biomass accumulation increased. Only the maximum biocide concentration (80 mg L⁻¹) resulted in a change in planktonic cell yields and temporal inhibition of biofilm activity and biomass, a factor that needs due consideration in view of dilution in industrial settings. Interfacing the real-time measurement of metabolic activity and biomass with dosing systems is especially relevant to optimizing the use of biocides in industrial water systems.

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在连续流动条件下，尽管接触了异噻唑啉酮，混合物种生物膜仍能发挥浮游细胞工厂的作用。

治理工业用水系统中生物污垢的主要方法是大规模使用杀菌剂。众所周知，生物膜是 "细胞工厂"，即使在抗菌剂的作用下也会释放浮游细胞。使用 CEMS-BioSpec 系统对异噻唑啉酮对混合假单胞菌生物膜的代谢活性和生物量的影响进行了实时监测。生物膜暴露在最低抑制浓度（1.25 mg L-1）的杀菌剂中不会影响浮游细胞的产生（对数 7.5 CFU mL-1），而整个生物膜的代谢活性和生物量积累则有所增加。只有最大杀菌剂浓度（80 毫克/升-1）才会导致浮游细胞产量的变化以及生物膜活性和生物量的暂时性抑制，考虑到工业环境中的稀释问题，需要适当考虑这一因素。将代谢活动和生物量的实时测量与加药系统结合起来，对于优化工业用水系统中杀菌剂的使用尤为重要。

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来源期刊

Environmental Microbiology Reports ENVIRONMENTAL SCIENCES-MICROBIOLOGY

CiteScore

6.00

自引率

3.00%

发文量

审稿时长

3.0 months

期刊介绍： The journal is identical in scope to Environmental Microbiology, shares the same editorial team and submission site, and will apply the same high level acceptance criteria. The two journals will be mutually supportive and evolve side-by-side. Environmental Microbiology Reports provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens.