Jagandeep S Saraya, Scott R Sammons, Derek K O'Flaherty
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引用次数: 0
Abstract
Nucleic acid conjugation methodologies involve linking the nucleic acid sequence to other (bio)molecules covalently. This typically allows for nucleic acid property enhancement whether it be for therapeutic purposes, biosensing, etc. Here, we report a streamlined, aqueous compatible, on-column conjugation methodology using nucleic acids containing a site-specific amino-modifier. Both monophosphates and carboxylates were amenable to the conjugation strategy, allowing for the introduction of a variety of useful handles including azide, aryl, and hydrophobic groups in DNA. We find that an on-column approach is superior to post-synthetic template-directed synthesis, mainly with respect to product purification and recovery.
核酸共轭方法涉及将核酸序列与其他(生物)分子共价连接。这通常可以提高核酸的性能,无论是用于治疗目的还是生物传感等。在此,我们报告了一种使用含有位点特异性氨基修饰剂的核酸的简化水相柱上共轭方法。单磷酸酯和羧酸酯都适用于该共轭策略,从而可以在 DNA 中引入叠氮、芳基和疏水基团等各种有用的处理。我们发现,柱上方法优于合成后模板指导合成,主要是在产品纯化和回收方面。