Protective Effect of Fasudil on Testicular Ischemia-Reperfusion Injury in Rats.

IF 4.7 2区医学 Q1 CHEMISTRY, MEDICINAL

Drug Design, Development and Therapy Pub Date : 2024-09-25 eCollection Date: 2024-01-01 DOI:10.2147/DDDT.S480774

Cem Kaya, Alparslan Kapisiz, Sibel Eryilmaz, Ramazan Karabulut, Zafer Turkyilmaz, Mehmet Arda Inan, Gizem Yaz Aydin, Kaan Sonmez

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引用次数: 0

Abstract

Background: Ischemia-reperfusion (I/R) injury to the testis can lead to organ damage, infertility, and subfertility. The goal of this study was to investigate the effects of fasudil on this devastating condition.

Methods: Thirty male Long-Evans rats were divided into five groups: a control group (no torsion), rats administered fasudil (30 mg/kg, no torsion), rats subject to ischemia with no treatment (I) (I/R injury), injured rats that received treatment 1 (T1) (I/R with 30 mg/kg fasudil before detorsion), and injured rats that received treatment 2 (T2) (I/R with 30 mg/kg fasudil after detorsion). Serum levels of TNF-ɑ and IL-6, along with tissue levels of glutathione (GSH), malondialdehyde (MDA), caspase-3, and Johnsen Tubular Biopsy Score (JTBS), were measured.

Results: Group I exhibited significantly higher levels of MDA and caspase-3 than all other groups except T2 (p ˂ 0.05). Although the difference was not statistically significant, Group T2 exhibited lower MDA and caspase-3 levels than Group I (p ˃ 0.05). Additionally, Group I displayed significantly higher TNF-ɑ and IL-6 levels, and lower GSH and JTBS values, than the other groups (p ˂ 0.05).

Conclusion: Our findings indicate that fasudil protects the testis from I/R injury, particularly when administered early.

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法舒地尔对大鼠睾丸缺血再灌注损伤的保护作用

背景：睾丸缺血再灌注（I/R）损伤可导致器官损伤、不育和亚育。本研究旨在探讨法舒地尔对这种破坏性疾病的影响：方法：将30只雄性Long-Evans大鼠分为5组：对照组（无扭转）、服用法舒地尔（30毫克/千克，无扭转）的大鼠、未接受任何治疗的缺血大鼠（I）（I/R损伤）、接受治疗1的损伤大鼠（T1）（扭转前用30毫克/千克法舒地尔进行I/R）和接受治疗2的损伤大鼠（T2）（扭转后用30毫克/千克法舒地尔进行I/R）。测量血清中 TNF-ɑ 和 IL-6 的水平，以及组织中谷胱甘肽 (GSH)、丙二醛 (MDA)、Caspase-3 和约翰森肾小管活检评分 (JTBS) 的水平：结果：除 T2 组外，I 组的 MDA 和 caspase-3 水平明显高于其他各组（P ˂ 0.05）。虽然差异不具有统计学意义，但 T2 组的 MDA 和 caspase-3 水平低于 I 组（p ˃ 0.05）。此外，I组的TNF-ɑ和IL-6水平明显高于其他组，而GSH和JTBS值则低于其他组（p ˂ 0.05）：我们的研究结果表明，法舒地尔能保护睾丸免受I/R损伤，尤其是在早期给药时。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Drug Design, Development and Therapy CHEMISTRY, MEDICINAL-PHARMACOLOGY & PHARMACY

CiteScore

9.00

自引率

0.00%

发文量

382

审稿时长

>12 weeks

期刊介绍： Drug Design, Development and Therapy is an international, peer-reviewed, open access journal that spans the spectrum of drug design, discovery and development through to clinical applications. The journal is characterized by the rapid reporting of high-quality original research, reviews, expert opinions, commentary and clinical studies in all therapeutic areas. Specific topics covered by the journal include: Drug target identification and validation Phenotypic screening and target deconvolution Biochemical analyses of drug targets and their pathways New methods or relevant applications in molecular/drug design and computer-aided drug discovery* Design, synthesis, and biological evaluation of novel biologically active compounds (including diagnostics or chemical probes) Structural or molecular biological studies elucidating molecular recognition processes Fragment-based drug discovery Pharmaceutical/red biotechnology Isolation, structural characterization, (bio)synthesis, bioengineering and pharmacological evaluation of natural products** Distribution, pharmacokinetics and metabolic transformations of drugs or biologically active compounds in drug development Drug delivery and formulation (design and characterization of dosage forms, release mechanisms and in vivo testing) Preclinical development studies Translational animal models Mechanisms of action and signalling pathways Toxicology Gene therapy, cell therapy and immunotherapy Personalized medicine and pharmacogenomics Clinical drug evaluation Patient safety and sustained use of medicines.