Immune cell kinetics after allogeneic red blood cell transfusion in patients undergoing cardiovascular surgery

IF 1.4 4区医学 Q4 HEMATOLOGY

Transfusion Clinique et Biologique Pub Date : 2024-09-24 DOI:10.1016/j.tracli.2024.09.002

Marie Yamada , Mami Nakao , Naotomo Yamada , Hideaki Nakamura , Manabu Itoh , Junji Yunoki , Keiji Kamohara , Shinya Kimura , Eisaburo Sueoka

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引用次数: 0

Abstract

Background and Objectives

Recent reports have highlighted that allogeneic blood transfusions decrease immune responses and affect patient outcomes. However, the effects of allogeneic red blood cell transfusions on the composition of immune cells are unclear. We aimed to clarify the alterations in host immune cells in patients who received allogeneic red blood cell transfusions during the perioperative period of cardiovascular surgery.

Materials and Methods

Eight non-transfused, 22 intraoperative autotransfusions, and 36 allogeneic red blood cell-transfused patients undergoing surgery were grouped, and lymphocyte subsets were analyzed using flow cytometry. Blood samples collected before surgery, approximately 1-week, and 1-month after surgery were used for analysis. Surgical parameters, operation time, blood loss, and length of hospital stay were also assessed.

Results

The group receiving transfusions showed statistical significance compared to non-transfused in the above-mentioned surgical parameters. When comparing the autologous and allogeneic transfusion groups, only the allogeneic red blood transfusion group had a longer hospital stay. In comparing preoperative and 1-week and 1-month postoperative samples, there were almost no differences in CD4, CD20, or NK counts between the autotransfusions and the allogenic red blood cell transfusion groups. In contrast, a significant decrease in lymphocyte count was observed in the allogenic red blood cell transfused group 1-week postoperatively compared to preoperatively. Moreover, the number of CD8 + cells was statistically lowest in the allogeneic transfusion group 1 week after the operation.

Conclusion

Our results suggest that allogeneic red blood cell transfusion could alter immune cell composition especially CD8 + cells, potentially impacting immune function.

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心血管手术患者输注异体红细胞后的免疫细胞动力学。

背景和目的：最近的报告强调，异体输血会降低免疫反应并影响患者的预后。然而，异体红细胞输血对免疫细胞组成的影响尚不清楚。我们旨在明确在心血管手术围手术期接受异体红细胞输血的患者宿主免疫细胞的变化：对 8 名未输血、22 名术中自体输血和 36 名接受异体红细胞输血的手术患者进行分组，并使用流式细胞术分析淋巴细胞亚群。分析中使用了手术前、手术后约 1 周和 1 个月采集的血液样本。此外，还对手术参数、手术时间、失血量和住院时间进行了评估：结果：在上述手术参数中，接受输血组与未接受输血组相比具有统计学意义。比较自体输血组和异体输血组，只有异体输血组的住院时间更长。比较术前、术后 1 周和 1 个月的样本，自体输血组和异体红细胞输血组的 CD4、CD20 或 NK 计数几乎没有差异。相比之下，术后 1 周的异体红细胞输注组淋巴细胞计数比术前明显减少。此外，据统计，术后 1 周异体输血组的 CD8+ 细胞数量最少：我们的研究结果表明，异体红细胞输注可改变免疫细胞的组成，尤其是 CD8+ 细胞，从而对免疫功能产生潜在影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Transfusion Clinique et Biologique 医学-免疫学

CiteScore

2.50

自引率

11.80%

发文量

234

审稿时长

36 days

期刊介绍： Transfusion Clinique et Biologique, the official journal of the French Society of Blood Transfusion (SFTS): - an aid to training, at a European level - the only French journal indexed in the hematology and immunology sections of Current Contents Transfusion Clinique et Biologique spans fundamental research and everyday practice, with articles coming from both sides. Articles, reviews, case reports, letters to the editor and editorials are published in 4 editions a year, in French or in English, covering all scientific and medical aspects of transfusion: immunology, hematology, infectious diseases, genetics, molecular biology, etc. And finally, a convivial cross-disciplinary section on training and information offers practical updates. Readership: "Transfusers" are many and various: anesthetists, biologists, hematologists, and blood-bank, ICU and mobile emergency specialists...