Sero-evidence of Rickettsia Infection by ELISA in the Northern-Central Area of Bangladesh.

Mymensingh medical journal : MMJ Pub Date : 2024-10-01
F Ferdouse, M K Masud, F Ferdouse, M A W Sarker, T A B Islam, M Shormin, M A Hossain, S K Paul, N Kobayashi, S Ahmed
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Abstract

Detection of rickettsia most commonly done by simple, economical Weil-Felix test which detects IgM antibody. This initial investigation provides limited sound guidance to clinical decisions because of its low specificity and sensitivity. An alternative test, enzyme-linked immunosorbent assay (ELISA) is faster, less complicated, can also be automated. Advancements in molecular method like polymerase chain reaction (PCR) are highly specific, sensitive and rapid assays for detection of rickettsiales in many different samples including blood, tissue etc. This study was carried out to diagnose the rickettsial agent in the north-central (Mymensingh division) area of Bangladesh. In laboratory, we performed ELISA and PCR. The agent was diagnosed up to species level by molecular approach. A total of 150 febrile patients were included. All were clinically suspected cases of rickettsial fever attending inpatient and outpatient department of medicine and pediatrics of Mymensingh Medical College Hospital from Octy 2012 to January 2014. The laboratory tests were performed in Microbiology department of Mymensingh Medical College. Following universal safety precautions blood samples were collected, serum separated and both were stored at -20°C. IgM ELISA and Nested PCR were performed. Several genes by PCR were detected for confirmation of the presence of rickettsial agent in the blood. Among 150 clinically suspected cases 76(50.66%) were positive for ELISA, and 69(46.0%) were positive for PCR. The sensitivity and specificity of ELISA were 92.75% and 85.19% respectively taking PCR as gold standard. The prevalence of rickettsial infection found in this study was very much close to other countries of this Sub continent.

用酶联免疫吸附法检测孟加拉国中北部地区立克次体感染的血清证据。
检测立克次体最常用的方法是简单、经济的魏氏试验(Weil-Felix test),该试验检测IgM抗体。由于特异性和灵敏度较低,这种初步检测方法只能为临床决策提供有限的合理指导。另一种检测方法是酶联免疫吸附试验(ELISA),它更快、更简单,而且可以自动进行。聚合酶链式反应(PCR)等分子检测方法的发展是一种高度特异、灵敏和快速的检测方法,可用于检测血液、组织等不同样本中的立克次体。本研究旨在诊断孟加拉国中北部(迈门辛格省)地区的立克次体病原体。在实验室中,我们进行了 ELISA 和 PCR 检测。通过分子方法诊断出病原体的种类。共纳入 150 名发热患者。他们都是2012年10月至2014年1月期间在迈门辛医学院医院内科和儿科住院和门诊就诊的立克次体热临床疑似病例。实验室检测在迈门辛医学院微生物学系进行。按照通用安全预防措施采集血液样本,分离血清,并将两者保存在 -20°C 温度下。进行了 IgM ELISA 和巢式 PCR 检测。通过 PCR 检测多个基因,以确认血液中是否存在立克次体病原体。在150个临床疑似病例中,76人(50.66%)的ELISA检测结果呈阳性,69人(46.0%)的PCR检测结果呈阳性。以 PCR 作为金标准,ELISA 的灵敏度和特异度分别为 92.75% 和 85.19%。这项研究发现的立克次体感染率与亚欧大陆的其他国家非常接近。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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