NIR Laser Irradiation Promotes Osteogenic Differentiation of PDLSCs Through the Activation of TRPV1 Channels and Subsequent Calcium Signaling.

IF 1.8 Q2 SURGERY
Jia-Hao Zeng, Bing'er Ma, Xiao-Qing Shen, Yuan-Ming Geng
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Abstract

Near-infrared (NIR) irradiation has shown potential to stimulate osteogenic differentiation, but the mechanisms are not fully understood. The study is to investigate the effects of NIR laser irradiation on osteoblastic differentiation. Human periodontal ligament stem cells (hPDLSCs) were cultured in osteogenic medium and exposed to 810 nm NIR laser at 0.5 J/cm2 every 48 h. The transient receptor potential vanilloid (TRPV1) channel inhibitor capsazepine (CPZ) was used to evaluate the role of calcium influx. Osteogenic differentiation was assessed by proliferation (CCK-8), alkaline phosphatase (ALP) activity, mineralization (Alizarin Red), and expression of bone markers by PCR and Western blot over 2 weeks. Intracellular calcium was measured by Fluo-4M dye and flow cytometry. Results showed that NIR irradiation enhanced hPDLSC proliferation, ALP activity, mineralization, and bone marker expression, indicating increased osteogenic differentiation. These effects were inhibited by CPZ. NIR induced a transient rise in intracellular calcium peaking at 3 min, which was blocked by CPZ. In conclusion, this study demonstrates that NIR laser irradiation promotes osteogenic differentiation of PDLSCs through the activation of TRPV1 channels and subsequent calcium signaling. Further research is warranted to optimize the treatment parameters and elucidate the detailed signaling pathways involved, paving the way for the clinical application of NIR therapy in the treatment of bone disorders and periodontal disease.

近红外激光照射通过激活 TRPV1 通道和随后的钙信号转导促进 PDLSCs 的成骨分化
近红外(NIR)辐照具有刺激成骨细胞分化的潜力,但其机制尚不完全清楚。本研究旨在探讨近红外激光照射对成骨细胞分化的影响。将人牙周韧带干细胞(hPDLSCs)培养在成骨培养基中,每 48 小时暴露于 0.5 J/cm2 的 810 nm 近红外激光。在两周内,通过增殖(CCK-8)、碱性磷酸酶(ALP)活性、矿化(茜素红)以及通过 PCR 和 Western 印迹检测骨标志物的表达来评估成骨分化。细胞内钙通过 Fluo-4M 染料和流式细胞术进行测量。结果表明,近红外照射增强了 hPDLSC 的增殖、ALP 活性、矿化和骨标记表达,表明成骨分化增强。CPZ 可抑制这些效应。近红外诱导细胞内钙一过性升高,在 3 分钟达到峰值,CPZ 可阻断这种升高。总之,本研究表明,近红外激光照射通过激活 TRPV1 通道和随后的钙信号转导促进了 PDLSCs 的成骨分化。我们需要进一步研究,优化治疗参数,阐明其中涉及的详细信号通路,为近红外疗法在骨病和牙周病治疗中的临床应用铺平道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.10
自引率
0.00%
发文量
0
期刊介绍: Photobiomodulation, Photomedicine, and Laser Surgery Editor-in-Chief: Michael R Hamblin, PhD Co-Editor-in-Chief: Heidi Abrahamse, PhD
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