Selective On-Site Detection and Quantification of Polystyrene Microplastics in Water Using Fluorescence-Tagged Peptides and Electrochemical Impedance Spectroscopy
Abbas Motalebizadeh, Somayeh Fardindoost, Mina Hoorfar
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引用次数: 0
Abstract
In this study, we developed a method for the on-site selective detection and quantification of microplastics in various water matrices using fluorescence-tagged peptides combined with electrochemical impedance spectroscopy (EIS). Among the types of plastics found in seawater, polystyrene (PS) microplastics were selected. Fluorometry, scanning electron microscopy (SEM), and Raman spectroscopy were used to verify the specific interaction of these peptides with PS spherical particles of different sizes (ranging from 0.1 to 250 µm). Principal component analysis (PCA) was employed to determine the effects of temperature (25-65 °C), incubation time (5 and 10 minutes), and particle size on plastic-peptide bonding efficiency, based on fluorescence intensity. For each water type (pure, tap, NaCl (0.5 M), and seawater), EIS plots (Nyquist and Bode) were generated. Significant factors affecting the EIS response, including particle size, shape, and material, were analyzed by measuring electrical parameters for different microplastic concentrations (50 ppb to 20 ppm). The EIS parameters changed with increasing plastic concentration, determining a limit of detection (LOD) of 50 ppb (ng/mL) for pure and tap water and 400 ppb for saline water, as the lowest concentration producing a significant change in EIS parameters compared to the baseline. The sensor proved highly effective for detecting microplastics in low ionic strength environments such as pure and tap water. However, in high ionic strength environments like saline and seawater, the detection capability diminished, likely due to the masking effect of ions on the EIS response.
期刊介绍:
The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.