Regime difference between macrophyte and Cyanophyta dominance regulate microbial carbon sequestration mode in lake sediments

Abstract

Different nutrient load in the lake ecosystems trigger the regime difference and change of predominant biotype. The regulation of carbon (C) sequestration mode in the lacustrine sediments in response to this process need prudent clarification. Fluorescence measurements and high-throughput sequencing for functional genes cbbL and cbbM encoding C-fixing bacteria genus were executed for sediments from two representative regimes dominated by macrophyte and Cyanophyta, respectively. The results showed that humic-like and fulvic-acid like materials dominated the dissolved organic matter (DOM) from the algae and macrophyte-dominated lake regions, respectively. Microbial assimilation played critical influence on C fixation into the sediments in both of the two regimes. However, higher diversity was detected in macrophyte-dominated regime compared to that in Cyanophyta-dominated regime, suggesting that moderate nutrient levels facilitated the species richness of bacteria encoding functional genes concerning C fixation. Bacterial species and diversities varied between two regimes including predominant and rare taxa, suggesting that community structure alteration due to regime difference triggered the regulation the C sequestration mode and stability. Predominant genera manipulated the abundance of C-fixing bacteria genes in response to the regulation of nutrient levels. Noted that rare genera also responded to the regime difference and played key role in C sequestration into lacustrine sediments. Our results suggest that more abundant macrophyte-dominated regime facilitated the C sequestration in the lake ecosystems for atmospheric C reduction.