Development of Loop-Mediated Isothermal Amplification (LAMP) Kit for the Detection of Fusarium Wilt Using SIX8 Genes

IF 1.1 4区农林科学 Q3 PLANT SCIENCES

Journal of Phytopathology Pub Date : 2024-09-27 DOI:10.1111/jph.13398

Perry Lorraine Duran Canare, Roselyn Jove Uy, Shinju Konishi, Hana Tonami, Dionisio G. Alvindia, Chiharu Nakashima

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Abstract

Panama disease, or Fusarium wilt, is a soil-borne fungal disease of Musa spp. and the most severe threat to the banana industry and food security worldwide with no cure. Therefore, early detection of the pathogen Fusarium oxysporum f. sp. cubense (Foc) through reliable diagnostics will aid in implementing plant quarantine and containment measures to prevent the spread of this disease. In this study, a primary diagnostic procedure was developed based on the DNA sequences of the Foc-TR4 SIX8a homologue, extracted from a piece of pseudostem tissue of M. acuminata Gros Michel Subgroup from Japan. A set of primers for the loop-mediated isothermal amplification (LAMP) reaction was designed. The developed specific marker can be used to accurately, rapidly, and reliably detect early diagnosis of Foc-TR4 infection without cutting down the unconfirmed young plant in banana fields.

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利用 SIX8 基因开发用于检测镰刀菌枯萎病的环路介导等温扩增（LAMP）试剂盒

巴拿马病或镰刀菌枯萎病是穆萨属植物的土传真菌病害，也是全球香蕉产业和食品安全面临的最严重威胁，目前尚无根治方法。因此，通过可靠的诊断方法及早发现病原体 Fusarium oxysporum f. sp. cubense（Foc）将有助于实施植物检疫和遏制措施，防止这种疾病的蔓延。本研究根据从日本 M. acuminata Gros Michel 亚群假茎组织中提取的 Foc-TR4 SIX8a 同源物的 DNA 序列，开发了一种初级诊断程序。设计了一套用于环介导等温扩增（LAMP）反应的引物。所开发的特异性标记可用于准确、快速、可靠地检测 Foc-TR4 感染的早期诊断，而无需砍掉香蕉田中未经确认的幼苗。

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来源期刊

Journal of Phytopathology 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.