An alternative method for comparing hemorrhagic activity of snake venoms following one of the 3R's principles

IF 2.6 4区医学 Q2 PHARMACOLOGY & PHARMACY

Toxicon Pub Date : 2024-09-26 DOI:10.1016/j.toxicon.2024.108111

Lídia Jorge Tasima , Victor Koiti Kavazoi , Eduardo Oliveira Venancio de Lima , Kathleen Fernandes Grego , Anita Mitico Tanaka-Azevedo

引用次数: 0

Abstract

Venom-induced hemorrhage analysis usually is performed by Minimum Hemorrhagic Dose (MHD), however a similar method can be used to compare venoms with fewer laboratory animals. Our work compared the MHD of five different venoms, with the size of hemorrhagic spot, finding good correlations in the results. Considering the 3Rs principle, we propose the use of the hemorrhagic spot method to compare hemorrhagic activity of snake venoms, rather than using the MHD method, since the first one needs 5 times less animals than the other.

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按照 3R 原则之一比较蛇毒出血活性的另一种方法

毒液引起的出血分析通常是通过最小出血剂量（MHD）来进行的，但类似的方法也可用于比较实验动物数量较少的毒液。我们的研究比较了五种不同毒液的最小出血剂量与出血点的大小，发现结果之间存在良好的相关性。考虑到 3Rs 原则，我们建议使用出血点法来比较蛇毒的出血活性，而不是使用 MHD 法，因为前者所需的动物比后者少 5 倍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicon 医学-毒理学

CiteScore

4.80

自引率

10.70%

发文量

358

审稿时长

68 days

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