The role of CD24hiCD27+ regulatory B cells in human chronic rhinosinusitis with/without nasal polyps

IF 2.5 4区医学 Q3 IMMUNOLOGY

Immunobiology Pub Date : 2024-09-16 DOI:10.1016/j.imbio.2024.152854

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引用次数: 0

Abstract

Background

Regulatory B cells (Bregs) reduce allergic and autoimmune inflammation. However, their role in chronic rhinosinusitis (CRS) remains unknown. This study investigated the frequency and function of Breg subsets in the peripheral blood of patients with CRS.

Methods

The demographic and clinical characteristics were compared among control, CRSsNP, neCRSwNP, and eCRSwNP groups. The expression of various Breg subtypes was evaluated in peripheral blood mononuclear cells (PBMCs) of patients with eosinophilic CRS with nasal polyps (eCRSwNP), non-eosinophilic CRS with nasal polyps (neCRSwNP), CRS without nasal polyps (CRSsNP). CD19⁺CD24^hiCD27⁺ B cells (B10 cells) were isolated by flow cytometry, followed by RNA sequencing (RNA-seq). Finally, IL-10 secreted by B10 cells were evaluated through the intracellular stain.

Results

A higher number of eosinophils in peripheral blood and nasal polyps were found in eCRSwNP compared with neCRSwNP, CRSsNP, and control groups. The frequency of B10 in the peripheral blood B cells (B10%) of patients with eCRSwNP was significantly lower than that in the neCRSwNP and control groups. B10% was negatively correlated with the quantity of tissue eosinophils, and the percentage and absolute value of peripheral blood eosinophils. The eCRSwNP, neCRSwNP and control groups had 1403 differentially expressed genes, 35 of which were identified in four highly enriched pathways. Additionally, the frequency of IL-10⁺B10 cells in peripheral blood was lower in patients with eCRSwNP than in the neCRSwNP and control groups.

Conclusion

This study is the first to reveal differences in both the quantity and IL-10 secretion of B10 cells in patients with eCRSwNP and neCRSwNP. These variations were strongly negatively associated with eosinophils in nasal polyps and peripheral blood. IL-10⁺B10 cells may play a key role in the pathological mechanisms of CRS, particularly the recurrence of eCRSwNP.

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CD24hiCD27+ 调节性 B 细胞在伴有/不伴有鼻息肉的人类慢性鼻窦炎中的作用

背景调节性 B 细胞（Bregs）能减轻过敏性和自身免疫性炎症。然而，它们在慢性鼻炎（CRS）中的作用仍然未知。本研究调查了CRS患者外周血中Breg亚群的频率和功能。方法比较了对照组、CRSsNP组、neCRSwNP组和eCRSwNP组的人口统计学和临床特征。方法比较了对照组、CRSsNP 组、neCRSwNP 组和 eCRSwNP 组患者的人口统计学和临床特征，评估了嗜酸性粒细胞性 CRS 伴鼻息肉（eCRSwNP）、非嗜酸性粒细胞性 CRS 伴鼻息肉（neCRSwNP）和无鼻息肉性 CRS（CRSsNP）患者外周血单核细胞（PBMC）中各种 Breg 亚型的表达情况。通过流式细胞术分离 CD19+CD24hiCD27+ B 细胞（B10 细胞），然后进行 RNA 测序（RNA-seq）。结果与 neCRSwNP、CRSsNP 和对照组相比，eCRSwNP 组外周血和鼻息肉中嗜酸性粒细胞的数量更高。eCRSwNP 患者外周血 B 细胞中 B10 的频率（B10%）明显低于 neCRSwNP 组和对照组。B10% 与组织嗜酸性粒细胞的数量、外周血嗜酸性粒细胞的百分比和绝对值呈负相关。eCRSwNP组、neCRSwNP组和对照组共有1403个差异表达基因，其中35个基因在四条高度富集的通路中被确定。此外，eCRSwNP 患者外周血中 IL-10+B10 细胞的频率低于 neCRSwNP 组和对照组。这些变化与鼻息肉和外周血中的嗜酸性粒细胞呈强负相关。IL-10+B10 细胞可能在 CRS 的病理机制中发挥关键作用，尤其是在 eCRSwNP 的复发中。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Immunobiology 医学-免疫学

CiteScore

5.00

自引率

3.60%

发文量

108

审稿时长

55 days

期刊介绍： Immunobiology is a peer-reviewed journal that publishes highly innovative research approaches for a wide range of immunological subjects, including • Innate Immunity, • Adaptive Immunity, • Complement Biology, • Macrophage and Dendritic Cell Biology, • Parasite Immunology, • Tumour Immunology, • Clinical Immunology, • Immunogenetics, • Immunotherapy and • Immunopathology of infectious, allergic and autoimmune disease.