Impact of FokI (rs2228570) and BglI (rs739837) polymorphisms in VDR gene on permanent tooth eruption: A cross-sectional study

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2024-09-27 DOI:10.1016/j.jobcr.2024.09.012

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Abstract

Introduction

Genetic polymorphisms who disturb the mineral homeostasis during tooth development and eruption are candidate to clarify the molecular mechanisms involved in changes in the tooth eruption chronology. In this study, we evaluate whether the FokI (rs2228570) and BglI (rs739837) polymorphisms in the Vitamin D receptor (VDR) gene are associated with changes in the chronology of eruption of permanent teeth.

Material & method

This cross-sectional study randomly included 353 biologically unrelated children, both sexes, without systemic impairment or syndromes and history of trauma during the primary dentition. One operator perform the oral clinical examination. The tooth was considered erupted if there was a visible minimum of any tooth surface emerging from the mucosa. Genomic DNA was extracted from buccal epithelial cells from saliva samples. Genotyping was performed by Real-Time Polymerase Chain Reactions using TaqMan® technology. The average of the total number of erupted permanent teeth between the genotypes was compared by the Mann-Whitney test and multivariate Generalized Linear Models (GLM) (α = 5 %). β values with Confidence Interval (CI) 95 % were calculated.

Results

The heterozygous adenine-guanine genotype of the FokI significantly decreases the number of erupted permanent teeth (β = −1.15; CI 95 % = −2.22 to −0.07; p = 0.036). In the stratified analysis for maxillary and mandibular teeth, this genotype was associated with a decrease in the number of erupted maxillary permanent teeth (β = −0.65; CI 95 % = −1.22 to −0.09; p = 0.023). BglI was not associated with permanent teeth eruption.

Conclusion

The FokI, but not BglI, in the VDR may delay the eruption of permanent teeth.

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VDR 基因中的 FokI (rs2228570) 和 BglI (rs739837) 多态性对恒牙萌出的影响：一项横断面研究

引言在牙齿发育和萌出过程中，干扰矿物质平衡的基因多态性是阐明牙齿萌出时序变化的分子机制的候选基因。在这项研究中，我们评估了维生素 D 受体（VDR）基因中的 FokI（rs2228570）和 BglI（rs739837）多态性是否与恒牙萌出时序的变化有关。由一名操作员进行口腔临床检查。如果牙齿表面有可见的最小部分露出粘膜，则认为牙齿已经萌出。从唾液样本的颊上皮细胞中提取基因组 DNA。基因分型采用 TaqMan® 实时聚合酶链反应技术进行。通过曼-惠特尼检验和多变量广义线性模型（GLM）（α = 5 %）比较了不同基因型之间萌出恒牙总数的平均值。结果FokI的杂合子腺嘌呤-鸟嘌呤基因型会显著减少萌出恒牙的数量（β = -1.15; CI 95 % = -2.22 to -0.07; p = 0.036）。在上颌和下颌牙齿的分层分析中，该基因型与上颌恒牙萌出数量的减少有关（β = -0.65；CI 95 % = -1.22 至 -0.09；p = 0.023）。结论 VDR 中的 FokI（而非 BglI）可能会延迟恒牙萌出。

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来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.