{"title":"A novel in vivo genome editing doubled haploid system for Zea mays L.","authors":"Huaxun Ye, Mei Louden, Jon A. T. Reinders","doi":"10.1038/s41477-024-01795-9","DOIUrl":null,"url":null,"abstract":"Doubled haploid (DH) technologies accelerate maize inbred development. Recently, methods using CRISPR–Cas have created gene-edited maize DH populations, albeit with relatively low editing frequencies. Restoring fertility via haploid chromosome doubling remains a critically important production constraint. Thus, improved editing and chromosome doubling outcomes are needed. Here we obtained maternally derived diploid embryos in vivo by ectopically co-expressing Zea mays BABY BOOM and cyclin D-like gene products within unfertilized egg cells. When combined with gene editing, the in vivo method enables the production of mature seed with a maternally derived, gene-edited diploid embryo without requiring in vitro tissue culture methods nor the use of a chemical chromosome doubling agent. In summary, we report a novel approach for creating gene-edited maize DH populations that we expect can accelerate genetic gain in a scalable, cost-effective manner. Ye et al. use a novel genetic haploid genome doubling method with parthenogenesis and gene editing to produce edited, maternally derived di-haploid progeny. A truncated BABY BOOM peptide confers both parthenogenesis and haploid genome doubling.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"10 10","pages":"1493-1501"},"PeriodicalIF":15.8000,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature Plants","FirstCategoryId":"99","ListUrlMain":"https://www.nature.com/articles/s41477-024-01795-9","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Doubled haploid (DH) technologies accelerate maize inbred development. Recently, methods using CRISPR–Cas have created gene-edited maize DH populations, albeit with relatively low editing frequencies. Restoring fertility via haploid chromosome doubling remains a critically important production constraint. Thus, improved editing and chromosome doubling outcomes are needed. Here we obtained maternally derived diploid embryos in vivo by ectopically co-expressing Zea mays BABY BOOM and cyclin D-like gene products within unfertilized egg cells. When combined with gene editing, the in vivo method enables the production of mature seed with a maternally derived, gene-edited diploid embryo without requiring in vitro tissue culture methods nor the use of a chemical chromosome doubling agent. In summary, we report a novel approach for creating gene-edited maize DH populations that we expect can accelerate genetic gain in a scalable, cost-effective manner. Ye et al. use a novel genetic haploid genome doubling method with parthenogenesis and gene editing to produce edited, maternally derived di-haploid progeny. A truncated BABY BOOM peptide confers both parthenogenesis and haploid genome doubling.
期刊介绍:
Nature Plants is an online-only, monthly journal publishing the best research on plants — from their evolution, development, metabolism and environmental interactions to their societal significance.