Dynamic in situ detection in iRhizo-Chip reveals diurnal fluctuations of Bacillus subtilis in the rhizosphere.

IF 11.3 1区化学 Q1 CHEMISTRY, PHYSICAL

ACS Catalysis Pub Date : 2024-09-26 DOI:10.1073/pnas.2408711121

Hengyi Dai,Binbin Wu,Yajuan Zhuang,Hao Ren,Yanbo Chen,Fangzhou Zhang,Chiheng Chu,Xiaofei Lv,Jianming Xu,Bin Ma

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Abstract

Effective colonization by microbe in the rhizosphere is critical for establishing a beneficial symbiotic relationship with the host plant. Bacillus subtilis, a soil-dwelling bacterium that is commonly found in association with plants and their rhizosphere, has garnered interest for its potential to enhance plant growth, suppress pathogens, and contribute to sustainable agricultural practices. However, research on the dynamic distribution of B. subtilis within the rhizosphere and its interaction mechanisms with plant roots remains insufficient due to limitations in existing in situ detection methodologies. To achieve dynamic in situ detection of the rhizosphere environment, we established iRhizo-Chip, a microfluidics-based platform. Using this device to investigate microbial behavior within the rhizosphere, we found obvious diurnal fluctuations in the growth of B. subtilis in the rhizosphere. Temporal dynamic analysis of rhizosphere dissolved oxygen (DO), pH, dissolved organic carbon, and reactive oxygen species showed that diurnal fluctuations in the growth of B. subtilis are potentially related to a variety of environmental factors. Spatial dynamic analysis also showed that the spatial distribution changes of B. subtilis and DO and pH were similar. Subsequently, through in vitro control experiments, we proved that rhizosphere DO and pH are the main driving forces for diurnal fluctuations in the growth of B. subtilis. Our results show that the growth of B. subtilis is driven by rhizosphere DO and pH, resulting in diurnal fluctuations, and iRhizo-Chip is a valuable tool for studying plant rhizosphere dynamics.

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iRhizo 芯片的原位动态检测揭示了根瘤菌中枯草芽孢杆菌的昼夜波动。

微生物在根圈中的有效定殖对于与寄主植物建立有益的共生关系至关重要。枯草芽孢杆菌（Bacillus subtilis）是一种生活在土壤中的细菌，通常与植物及其根瘤菌层结合在一起，因其具有促进植物生长、抑制病原体和促进可持续农业实践的潜力而备受关注。然而，由于现有原位检测方法的局限性，有关枯草芽孢杆菌在根圈中的动态分布及其与植物根系相互作用机制的研究仍然不足。为了实现对根圈环境的原位动态检测，我们建立了一个基于微流控技术的平台--iRhizo-Chip。利用该装置研究根圈中的微生物行为，我们发现根圈中枯草芽孢杆菌的生长有明显的昼夜波动。对根圈溶解氧（DO）、pH值、溶解有机碳和活性氧的时间动态分析表明，枯草芽孢杆菌生长的昼夜波动可能与多种环境因素有关。空间动态分析还表明，枯草芽孢杆菌的空间分布变化与溶解氧和 pH 值的变化相似。随后，通过离体对照实验，我们证明根瘤菌溶解氧和pH值是导致枯草芽孢杆菌生长昼夜波动的主要驱动力。我们的研究结果表明，枯草芽孢杆菌的生长受根圈溶解氧和酸碱度的驱动，从而导致昼夜波动，iRhizo-Chip 是研究植物根圈动力学的重要工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Catalysis CHEMISTRY, PHYSICAL-

CiteScore

20.80

自引率

6.20%

发文量

1253

审稿时长

1.5 months

期刊介绍： ACS Catalysis is an esteemed journal that publishes original research in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. It offers broad coverage across diverse areas such as life sciences, organometallics and synthesis, photochemistry and electrochemistry, drug discovery and synthesis, materials science, environmental protection, polymer discovery and synthesis, and energy and fuels. The scope of the journal is to showcase innovative work in various aspects of catalysis. This includes new reactions and novel synthetic approaches utilizing known catalysts, the discovery or modification of new catalysts, elucidation of catalytic mechanisms through cutting-edge investigations, practical enhancements of existing processes, as well as conceptual advances in the field. Contributions to ACS Catalysis can encompass both experimental and theoretical research focused on catalytic molecules, macromolecules, and materials that exhibit catalytic turnover.