Enzymatic synthesis of azide by a promiscuous N-nitrosylase

IF 9.6 1区化学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

ACS Materials Letters Pub Date : 2024-09-27 DOI:10.1038/s41557-024-01646-2

Antonio Del Rio Flores, Rui Zhai, David W. Kastner, Kaushik Seshadri, Siyue Yang, Kyle De Matias, Yuanbo Shen, Wenlong Cai, Maanasa Narayanamoorthy, Nicholas B. Do, Zhaoqiang Xue, Dunya Al Marzooqi, Heather J. Kulik, Wenjun Zhang

引用次数: 0

Abstract

Azides are energy-rich compounds with diverse representation in a broad range of scientific disciplines, including material science, synthetic chemistry, pharmaceutical science and chemical biology. Despite ubiquitous usage of the azido group, the underlying biosynthetic pathways for its formation remain largely unknown. Here we report the characterization of an enzymatic route for de novo azide construction. We demonstrate that Tri17, a promiscuous ATP- and nitrite-dependent enzyme, catalyses organic azide synthesis through sequential N-nitrosation and dehydration of aryl hydrazines. Through biochemical, structural and computational analyses, we further propose a plausible molecular mechanism for azide synthesis that sets the stage for future biocatalytic applications and biosynthetic pathway engineering.

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杂合 N-亚硝基酶酶促合成叠氮化物

叠氮化物是一种富含能量的化合物，在材料科学、合成化学、制药科学和化学生物学等广泛的科学学科中具有不同的代表性。尽管叠氮基的使用无处不在，但其形成的基本生物合成途径在很大程度上仍然未知。在这里，我们报告了从头构建叠氮基的酶学途径的特征。我们证明 Tri17 是一种依赖 ATP 和亚硝酸盐的杂合酶，它通过芳基肼的 N-亚硝基化和脱水顺序催化有机叠氮化物的合成。通过生化、结构和计算分析，我们进一步提出了叠氮化物合成的合理分子机制，为未来的生物催化应用和生物合成途径工程奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Materials Letters MATERIALS SCIENCE, MULTIDISCIPLINARY-

CiteScore

14.60

自引率

3.50%

发文量

261

期刊介绍： ACS Materials Letters is a journal that publishes high-quality and urgent papers at the forefront of fundamental and applied research in the field of materials science. It aims to bridge the gap between materials and other disciplines such as chemistry, engineering, and biology. The journal encourages multidisciplinary and innovative research that addresses global challenges. Papers submitted to ACS Materials Letters should clearly demonstrate the need for rapid disclosure of key results. The journal is interested in various areas including the design, synthesis, characterization, and evaluation of emerging materials, understanding the relationships between structure, property, and performance, as well as developing materials for applications in energy, environment, biomedical, electronics, and catalysis. The journal has a 2-year impact factor of 11.4 and is dedicated to publishing transformative materials research with fast processing times. The editors and staff of ACS Materials Letters actively participate in major scientific conferences and engage closely with readers and authors. The journal also maintains an active presence on social media to provide authors with greater visibility.