Morphological Characteristics, Genetic Diversity, and Microsatellite Analysis of the Progenies (F1, F2, and Backcross Descendant) of Siniperca chuatsi and Siniperca scherzeri

IF 1.9 4区农林科学 Q2 FISHERIES

Aquaculture Research Pub Date : 2024-09-26 DOI:10.1155/2024/6672569

Zhilun Zhang, Jijia Sun, Shuang Liu, Jiahui Chen, Xuchong Wei, Chenlei Huang, Li Lin, Qiuxian Chen, Xuange Liu, Haiyang Li, Huadong Yi, Sheng Bi, Linqiang Han, Jianhui Liang, Yong Zhang, Guifeng Li

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Abstract

Hybridization is a common technique in the breeding of new varieties. In this study, we obtained F₁ and F₂ hybrids through interspecific hybridization between Siniperca chuatsi and Siniperca scherzeri and backcross offspring by backcrossing between F₁ and Siniperca chuatsi. We analyzed the morphological traits and genetic diversity of the parents and their progeny. The results showed that although most morphological traits of the progeny were not significantly different from those of their parents, the F₁ hybrids exhibited the most pronounced morphological variations. The F₂ and backcross hybrids appeared morphological differentiation within their populations. The CPL/CPW ratio emerged as a reliable marker for identifying hybrids morphologically. The expected heterozygosity for F₁ (0.6264), F₂ (0.6677), and backcross offspring (0.5260) were intermediate between Siniperca chuatsi (0.7388) and Siniperca scherzeri (0.3910), indicating a relatively high level of genetic diversity in the offspring. Additionally, the private allele frequency of F₁ and F₂ was higher than backcross offspring. One SSR marker (G₅₂₂₀) was identified as a specific molecular marker to distinguish the hybrid F₁ population from the parents, hybrid F₂, and backcross F₁ populations by analyzing specific alleles. The result of Ne, He, I, and PIC confirmed that F₂ maintained the genetic diversity of F₁. We speculated that both F₁ and F₂ have higher breeding potential. This study provides essential baseline data for genetic breeding of mandarin fish that will be instrumental in the future.

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鳜鱼和鳜鱼后代（F1、F2 和回交后代）的形态特征、遗传多样性和微卫星分析

杂交是培育新品种的常用技术。在本研究中，我们通过鳜鱼和鳜鱼的种间杂交获得了 F1 和 F2 代杂交种，并通过 F1 和鳜鱼的回交获得了回交后代。我们分析了亲本及其后代的形态特征和遗传多样性。结果表明，虽然后代的大多数形态特征与亲本没有显著差异，但 F1 代杂交种的形态变异最为明显。F2 和回交杂交种在其群体内出现了形态分化。CPL/CPW 比值是鉴定杂种形态的可靠标记。F1（0.6264）、F2（0.6677）和回交后代（0.5260）的预期杂合度介于鳜鱼（0.7388）和鮨鱼（0.3910）之间，表明后代的遗传多样性水平相对较高。此外，F1 和 F2 的私有等位基因频率高于回交后代。通过分析特定等位基因，确定了一个 SSR 标记（G5220）作为特异性分子标记，用于区分杂交 F1 群体与亲本、杂交 F2 和回交 F1 群体。Ne、He、I和PIC的结果证实，F2保持了F1的遗传多样性。我们推测 F1 和 F2 都具有更高的育种潜力。这项研究为鸳鸯鱼的遗传育种提供了重要的基础数据，将在未来发挥重要作用。

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来源期刊

Aquaculture Research 农林科学-渔业

CiteScore

4.60

自引率

5.00%

发文量

464

审稿时长

5.3 months

期刊介绍： International in perspective, Aquaculture Research is published 12 times a year and specifically addresses research and reference needs of all working and studying within the many varied areas of aquaculture. The Journal regularly publishes papers on applied or scientific research relevant to freshwater, brackish, and marine aquaculture. It covers all aquatic organisms, floristic and faunistic, related directly or indirectly to human consumption. The journal also includes review articles, short communications and technical papers. Young scientists are particularly encouraged to submit short communications based on their own research.