Identification of Major Locus Conferring Resistance to Urdbean Leaf Crinkle Disease in Black Gram [Vigna mungo (L.) Hepper]

IF 1.1 4区农林科学 Q3 PLANT SCIENCES

Journal of Phytopathology Pub Date : 2024-09-26 DOI:10.1111/jph.13402

Sathya Palanivelu, Manivannan Narayana, Viswanathan Palaniappan

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引用次数: 0

Abstract

Urdbean leaf crinkle disease (ULCD) is a major factor causing severe yield loss in black gram production. The identification of quantitative trait loci (QTL) linked to ULCD resistance is highly useful in breeding programmes. A mapping population was developed from a cross between the resistant parent CO 5 and the susceptible parent TU 68. ULCD screening was carried out through the artificial sap inoculation method. In the F_2:3 population, the ULCD score was assessed 60 days after infection. Composite interval mapping was carried out with a 1000-run permutation. Another mapping population, VBN(Bg) 4 × CO 5 in F_2:3 generation was used to validate the identified QTL. A single locus, qULCD3.1 was identified in linkage group 3. It explains 17.98% of the variation in ULCD resistance. Markers VR086 and CEDG 305 flanked the QTL region. The marker CEDG 305 explains 20.5% of the phenotypic variation for ULCD resistance in the validation population VBN(Bg) 4 × CO 5. This is the first report to identify a QTL for ULCD disease resistance. The identified markers can be used to incorporate ULCD resistance in black gram through marker-assisted selection.

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鉴定黑禾苗[Vigna mungo (L.) Hepper]抗大豆叶片皱缩病的主要基因座

大豆皱叶病（ULCD）是导致黑糯米生产严重减产的一个主要因素。鉴定与抗ULCD相关的数量性状位点（QTL）对育种计划非常有用。由抗性亲本 CO 5 和易感亲本 TU 68 杂交培育出了一个制图群体。通过人工汁液接种法进行了ULCD筛选。在 F2:3 群体中，感染 60 天后对超低抗性进行评估。通过 1000 次排列进行了复合间隔作图。另一个F2:3代的作图群体VBN(Bg) 4 × CO 5被用来验证已鉴定的QTL。在第 3 连接组中发现了一个单基因座 qULCD3.1，该基因座解释了 17.98% 的 ULCD 抗性变异。标记 VR086 和 CEDG 305 位于 QTL 区域的侧翼。在验证群体 VBN(Bg) 4 × CO 5 中，标记 CEDG 305 解释了 20.5% 的抗ULCD 表型变异。这是首次发现抗ULCD病害的QTL。所鉴定的标记可用于通过标记辅助选择将 ULCD 抗性纳入黑糯米中。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Phytopathology 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.