Cloning and characterization of apolipoprotein A-IV (ApoA-IV) from Oreochromis niloticus involved in immune defense functions

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2024-09-26 DOI:10.1016/j.aqrep.2024.102355

Yongxiong Huang , Xuyan Tan , Jiamin Feng , Zhiqiang Zhang , Kwaku Amoaha , Berchie Asiedu , Yu Huang , Jichang Jian

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Abstract

Apolipoprotein A-IV (ApoA-IV) is a multifunctional protein that participates in a broad spectrum of biological processes, including the modulation of immune function. Nevertheless, the specific roles of ApoA-IV in the immune response to acute bacterial infections in Nile tilapia (Oreochromis niloticus) are yet to be fully elucidated. In the present study, we identified ApoA-IV from O. niloticus (On-ApoA-IV) and examined its role in bacterial infections. The open reading frame of On-ApoA-IV spanned 768 bp and encoded a sequence of 255 amino acids. Its transcriptional abundance was the highest in the intestine, and On-ApoA-IV was induced in tilapia challenged with Streptococcus agalactiae. Additionally, experimental findings indicated that On-ApoA-IV can inhibit inflammatory processes and apoptosis, thereby enhancing tilapia survival during acute bacterial infection. The current data provide a theoretical foundation for further elucidation of the mechanisms by which ApoA-IV safeguard fish against pathogens.

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参与免疫防御功能的黑线鲈载脂蛋白 A-IV (ApoA-IV) 的克隆和表征

载脂蛋白 A-IV（ApoA-IV）是一种多功能蛋白质，参与多种生物过程，包括调节免疫功能。然而，载脂蛋白 A-IV 在尼罗罗非鱼（Oreochromis niloticus）对急性细菌感染的免疫反应中的具体作用尚未完全阐明。在本研究中，我们鉴定了尼罗罗非鱼的载脂蛋白A-IV（On-ApoA-IV），并研究了它在细菌感染中的作用。On-ApoA-IV 的开放阅读框长达 768 bp，编码 255 个氨基酸序列。在肠道中，On-ApoA-IV的转录丰度最高，在罗非鱼受到半乳链球菌挑战时，On-ApoA-IV被诱导。此外，实验结果表明，On-ApoA-IV 能抑制炎症过程和细胞凋亡，从而提高罗非鱼在急性细菌感染中的存活率。目前的数据为进一步阐明载脂蛋白A-IV保护鱼类免受病原体感染的机制提供了理论基础。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.