Pickering emulsions stabilized by chitosan nanoparticles and loaded with clove essential oil as a green biopesticide: A case study on controlling fungal growth in oranges

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2024-09-27 DOI:10.1016/j.postharvbio.2024.113234

Maryam Mottaki , Seid Mahdi Jafari , Seyyed Morteza Hosseini , Alireza Sadeghi , Fatemeh Heydari-Delfard

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引用次数: 0

Abstract

The purpose of this research was to optimize the production of Pickering emulsions (PEs) stabilized by chitosan nanoparticles (CS-NPs) and containing clove essential oil (CEO) as a biopesticide against the fungal growth (Penicillium digitatum) in oranges. First, CS-NPs were synthesized and characterized to prepare PEs. The produced CS-NPs had an average size < 100 nm, contact angle < 90° and a positive zeta potential; CS-NPs were hydrophilic, which showed their ability to stabilize oil-in-water PEs. Second, PEs of CEO in water stabilized by CS-NPs were prepared at different levels of nanoparticles, CEO content, and homogenizer speeds; FTIR spectrum, morphology, size and stability of these PEs during storage were studied. The optimal conditions for the production of PEs included 0.055 % CS-NPs, 1 % CEO and 25,000 rpm homogenization. This PE had an encapsulation efficiency of 98 % and a gradual and appropriate release during the storage time. In the third phase, the antifungal activity of different concentrations of PEs compared with Xedamix (a commercial fungicide) was investigated with the aim of increasing the shelf life of oranges after harvesting. According to in vitro antifungal activity, minimum fungicidal concentration of the produced PEs against P. digitatum was equal to 2 %. Moreover, there was no significant difference between in situ inhibitory effects of PEs and Xedamix on the target fungus in spiked oranges.

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由壳聚糖纳米颗粒稳定并添加丁香精油的皮克林乳剂作为一种绿色生物农药：控制柑橘中真菌生长的案例研究

本研究的目的是优化壳聚糖纳米颗粒（CS-NPs）稳定的皮克林乳剂（PEs）的生产，并将含有丁香精油（CEO）的皮克林乳剂（PEs）作为一种生物杀虫剂，以防止橙子中真菌（数字青霉）的生长。首先，合成 CS-NPs 并对其进行表征，以制备 PEs。所制备的 CS-NPs 平均粒径为 100 nm，接触角为 90°，zeta 电位为正；CS-NPs 具有亲水性，这表明其具有稳定水包油 PEs 的能力。其次，在不同的纳米粒子含量、CEO 含量和均质器速度下制备了 CS-NPs 稳定的 CEO 水包油 PE；研究了这些 PE 的傅立叶变换红外光谱、形态、尺寸和储存稳定性。生产 PE 的最佳条件包括 0.055 % 的 CS-NPs、1 % 的 CEO 和 25,000 rpm 的匀浆。这种 PE 的封装效率高达 98%，并能在储存期间逐渐适当释放。在第三阶段，研究了不同浓度的聚乙烯与 Xedamix（一种商用杀真菌剂）相比的抗真菌活性，目的是延长橙子收获后的货架期。根据体外抗真菌活性，生产的 PE 对地衣芽孢杆菌的最低杀菌浓度为 2%。此外，PEs 和 Xedamix 对加标橙子中目标真菌的原位抑制作用没有明显差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.